1887

Abstract

SUMMARY: -Methyl-'-nitro--nitrosoguanidine (NTG), which is known to be a very effective mutagen in many systems, induces petite mutations when cells of are treated in acetate buffer. Fifty per cent or more of the survivors may be petite mutants. NTG is a real mutagen in the process since the number of mutants increases at short times of exposure to NTG although the total number of cells decreases. Growing cells are more susceptible to killing and mutation than are cells in buffer. The production of petites may cause difficulties when screening for rare auxotrophs.

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/content/journal/micro/10.1099/00221287-48-2-277
1967-08-01
2024-05-04
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References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N-nitroN-nitrosoguamdine in Escherichia colik 12. Biochem.biophys. Res. Commun. 18:788
    [Google Scholar]
  2. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. J. Am. chem. Soc. 70:4267
    [Google Scholar]
  3. Ephrussi B. 1953 Nucleo-Cytoplasmic Relations in Microorganisms Their Bearing on Cell Heredity and Differentiation, p. 13. London: Oxford University Press.;
    [Google Scholar]
  4. Greene M. O., Greenberg J. 1960; The activity of nitrosoguanidines against ascites tumors in mice. Cancer Res. 20:1166
    [Google Scholar]
  5. Lederberg J., Zinder N. 1948; Concentration of biochemical mutants of bacteria with penicillin. J. Am. chem. Soc. 70:4267
    [Google Scholar]
  6. Mandell J. D., Greenberg J. 1960; A new chemical for bacteria, 1-methyl-3-nitro-i-nitroso-guanidine. Biochem.biophys. Res. Commun. 3:575
    [Google Scholar]
  7. Marquardt H., Zimmermann F., Schwaier R. 1964; Die Wirkungkrebsauslosender Nitro-samine und Nitrosamide auf das Adenin-6.45-Rückmutatationssystem von Saccharomyces cerevisiae. Z. Vererbungslehre 95:82
    [Google Scholar]
  8. Megnet,R 1964; A method for the selection of auxotrophic mutants of the yeast Schizosaccharo-mycespombe. Experientia 20:320
    [Google Scholar]
  9. Megnet R. 1965; Screening of auxotrophic mutants of Schizosaccharomycespombe with 2-deoxyglucose. Mutation Res. 2:328
    [Google Scholar]
  10. Moat A. G., Peters N., Srb A. M. 1959; Selection and isolation of auxotrophic yeast mutants with the aid of antibiotics. J. Bad. 77:673
    [Google Scholar]
  11. Nagai S. 1959; Induction of the respiration-deficient mutation in yeast by various synthetic dyes. Science 130:1188
    [Google Scholar]
  12. Nagai S., Yanagishima N., Nagai H. 1961; Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bact. Revs. 25:404
    [Google Scholar]
  13. Ogur M., St John R., Nagai S. 1957; Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science 125:988
    [Google Scholar]
  14. Schatz G., Halsbrunner E., Tuppy H. 1964; Deoxyribonucleic acid associated with yeast mitochondria. Biochem.biophys. Res. Commun. 15:127
    [Google Scholar]
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