1887

Abstract

SUMMARY: No significant improvement has been made on the medium first described for the primary culture of by Bordet & Gengou in 1906. An explanation for this was sought in the experiments described, and it was found to be most probable that potato extract provides optimal concentrations of amino-acids and peptides required for growth. Although serum can be used to replace whole blood in this medium, the latter is essential for maximal growth; for that reason the retention of the term haemophilus is justified.

Many peptones, especially the more digested ones, inhibit growth of in concentrations normally used in bacteriological media. In the absence of peptone, meat extract agar can be used as the base of a satisfactory medium, especially when used in conjunction with horse instead of rabbit blood.

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1951-05-01
2024-05-15
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References

  1. Bailey J. H. 1933; A medium for the isolation of Bacillus pertussis. J. inf. Dis 52:94
    [Google Scholar]
  2. Balch H. H. 1942; Copper content of commercial peptones. J. Path. Bact 54:377
    [Google Scholar]
  3. Bordet J., Gengou O. 1906; Le microbe de la coqueluche. Ann. Inst. Pasteur 20:731
    [Google Scholar]
  4. Chievitz I., Meyer A. 1918; Eine Methode zur Frühdiagnose des Keuchhustens. Münch med. Wschr 2:729
    [Google Scholar]
  5. Gardner A. D., Leslie P. H. 1932; Early diagnosis of whooping-cough by the cough-droplet method. Lancet i:9
    [Google Scholar]
  6. Glass V. 1939a; The effect of blood digest and haem on the growth of C. diphtheriae. J. Path. Bact 49:549
    [Google Scholar]
  7. Glass V. 1939b; The effect of blood treated by heat, acid or alkali on the growth of C. diphtheriae. J. Path. Bact 48:507
    [Google Scholar]
  8. Groot E. H. 1946; Investigation into the biologically important amino-acids in potato protein in connection with its nutritive value. Parts I, II, III, IV and V. Arch, néerl. Physiol 28:277–323340–349357
    [Google Scholar]
  9. Hornibrook J. W. 1939; Cultivation of Phase I H. pertussis in a semi-synthetic medium. Pub. Hlth Rep., Wash 54:1847
    [Google Scholar]
  10. Hornibrook J. W. 1940; Nicotinic acid as a growth factor for H. pertussis. Proc. Soc. exp. Biol., N.Y 45:598
    [Google Scholar]
  11. Kleinschmidt H. 1931; Der Bordet-Gengousche Keuchhustenbacillus und der Pfeiffersche Influenzabacillus.. Klin. Wschr 2:1847
    [Google Scholar]
  12. Kristensen M. 1922 Investigations into the Occurrence and Classification of the Haemoglobinophilic Bacteria p. 211 Copenhagen: Levin and Munksgaard;
    [Google Scholar]
  13. lawson G. McL. 1939; Immunity Studies in pertussis. Amer. J. Hyg 29:119 section B
    [Google Scholar]
  14. Leslie P. H., Gardner A. D. 1931; The phases of Haemophilus pertussis. J. Hyg., Camb 31:423
    [Google Scholar]
  15. Mackie T. J., Mccartney J. E. 1949 Handbook of Practical Bacteriology, 8th ed. p. 183 Edinburgh: E. and S Livingstone Ltd;
    [Google Scholar]
  16. Madsen Th. 1925; Whooping cough. Its bacteriology, diagnosis, prevention and treatment. Boston med. surg. J 192:50
    [Google Scholar]
  17. O’Meara R. A. Q., Macsween J. C. 1937; The influence of copper in peptones on the growth of certain pathogens in peptone broth. J. Path. Bact 44:225
    [Google Scholar]
  18. Pollock M. R. 1947; The growth of H. pertussis on media without blood. Brit. J. exp. Path 28:295
    [Google Scholar]
  19. Sauer L. W., Hambrecht L. 1930; Whooping cough. Early diagnosis by the cough plate method. J. Amer. med. Ass 95:263
    [Google Scholar]
  20. Silverthorne N., Cameron C. 1942; Whooping cough. IV. Beef liver extract agar media for growth of H. pertussis. J. Pediat 20:16
    [Google Scholar]
  21. Straker E. A., Westwater J. S. 1937; The bacteriological diagnosis of whooping-cough. Lancet ii:565
    [Google Scholar]
  22. Sugare H., McLeod J. W. 1929; The bacteriological diagnosis of whooping-cough. Lancet ii:165
    [Google Scholar]
  23. Verwey W. F., Thiele E. H., Sage D. N., Schuchardt L. F. 1949; A simplified liquid culture medium for the growth of H. pertussis. J. Bact 58:127
    [Google Scholar]
  24. Wollstein M. 1909; The Bordet-Gengou bacillus of pertussis. J. exp. Med 11:41
    [Google Scholar]
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