1887

Abstract

SUMMARY: During utilization of [C]acetate by non-photosynthetic var. C was regularly found in trehalose, phosphate esters, glutamate, malate, fumarate, succinate, aspartate, alanine, -aminobutyrate, and six unidentified ethanol-soluble compounds. Of the unidentified substances only two, an anthrone-positive material and an ether-soluble substance, were quantitatively important. Small amounts of radioactive laminaribiose, lamin-aritriose, and what were probably higher oligosaccharides of the laminarin series were found by a more sensitive procedure, as well as several additional labelled ninhydrin-positive substances. The kinetics of labelling were consistent with oxidation of acetate via the tricarboxylic acid cycle and assimilation via the glyoxylate cycle and ‘reversed’ glycolysis. There was no indication of any qualitative difference between the intermediates of acetate metabolism and those of oxidation of endogenous reserves. Rapid fluctuations in levels of labelled intermediates were observed, some of which coincided with shoulders or plateaux in the radioactivity of the ethanol-soluble fraction as a whole.

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/content/journal/micro/10.1099/00221287-55-2-257
1969-02-01
2024-05-02
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References

  1. Betz A., Chance B. 1965; Phase relationship of glycolytic intermediates in yeast cells with oscillatory metabolic control. Archs Biochem. Biophys 109:585
    [Google Scholar]
  2. Danforth W. F. 1953; Oxidative metabolism of Euglena. Archs Biochem. Biophys 46:164
    [Google Scholar]
  3. Danforth W. F. 1961; Oxidative assimilation of acetate by Euglena. Carbon balance and effects of ethanol. J. Protozool 8:152
    [Google Scholar]
  4. Danforth W. F. 1967 In Research in Protozoology. Chen T.-T. Ed. by I p. 215 Oxford: Pergamon Press;
    [Google Scholar]
  5. Danforth W. F., Wilson B. W. 1957; Adaptive changes in the acetate metabolism of Euglena. J. Protozool 4:52
    [Google Scholar]
  6. Danforth W. F., Wilson B. W. 1961; The endogenous metabolism of Euglena gracilis . J. gen. Microbiol 24:95
    [Google Scholar]
  7. Elliott K. A. C. 1965; γ-Aminobutyric acid and other inhibitory substances. Br. med. Bull 21:70
    [Google Scholar]
  8. Goldemberg S. H., Marechal L. R., De Souza B. C. 1966; β-1,3-Oligoglucan: orthophosphate glucosyltransferase from Euglena gracilis. . J. biol. Chem. 241:45
    [Google Scholar]
  9. Hanes C. S., Isherwood F. A. 1949; Separation of the phosphoric esters on filter paper chromatograms. Nature; Lond: 1641107
    [Google Scholar]
  10. Marechal L. R., Goldemberg S. H. 1964; Uridine diphosphate glucose-β-1, 3-glucan-β-3-gluco- syltransferase from Euglena gracilis. . J. biol. Chem. 239:3163
    [Google Scholar]
  11. Marzullo G., Danforth W. F. 1964a; Kinetic studies on the oxidative assimilation of acetate by a non-photosynthetic strain of Euglena gracilis. . J. gen. Microbiol 34:9
    [Google Scholar]
  12. Marzullo G., Danforth W. F. 1964b; Composition of the ethanol-insoluble assimilatory products of oxidative assimilation of acetate by Euglena gracilis. . J. gen. Microbiol 34:21
    [Google Scholar]
  13. Mccalla D. R. 1963; Accumulation of extracellular amino acids by Euglena gracilis. . J. Protozool 10:491
    [Google Scholar]
  14. Warburg O. 1958; Photosynthesis. Science, N.Y 128:68
    [Google Scholar]
  15. Wilson B. W., Danforth W. F. 1958; The extent of acetate and ethanol oxidation by Euglena gracilis. . J. gen. Microbiol 18:535
    [Google Scholar]
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