1887

Abstract

Summary

The ultrastructure of cells of the sclerotia and aerial mycelium of Sacc. was studied by electron microscopy. A mature sclerotium of contains several types of differentiated cells. The thick-walled rind cells which comprise the sclerotial envelope are empty. The underlying cortex cells have thinner walls and contain many vesicles full of reserve materials which appear dark after treatment with osmium tetroxide. Although less reactive to osmium tetroxide, the cells between the cortex and medulla are also rich in reserve materials, some of which are membrane-bound. The inner layer (medulla) is composed of cells with extremely thick walls, of thinner-walled cells full of reserve materials and of empty cells. The walls of the hyphal cells are significantly thinner, and less optically dense than the walls of any of the sclerotial cells. Hyphal cells contain more ribosomes and mitochondria than sclerotial cells.

It seems that the resistance of sclerotia to biological degradation depends upon the melanin-rich rind as well as the wall structure and organization of cells comprising the inner layers of the sclerotium.

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/content/journal/micro/10.1099/00221287-57-2-143
1969-08-01
2024-04-28
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References

  1. Aycock R. 1966; Stem rot and other diseases caused by Sclerotium rolfsii. Tech. Bull174N. Carol, agric. Exp Stn. N.C. State University at Raleigh; N. C.:
    [Google Scholar]
  2. Bloomfield B. J., Alexander M. 1967; Melanins and resistance of fungi to lysis. J. Bact 93:1276
    [Google Scholar]
  3. Bracker C. E. 1967; Ultrastructure of fungi. A. Rev. Phytopath 5:343
    [Google Scholar]
  4. Butler G. M. 1966; Vegetative structures. In The Fungi Ainsworth G. C., Sussman A. S. 283 New York and London: Academic Press;
    [Google Scholar]
  5. Calonge F. D., Fielding A. H., Byrde R. J. W. 1969; Multivesicular bodies in Sclerotinia fructigena and their possible relation to extracellular enzyme secretion. J. gen. Microbiol 55:177
    [Google Scholar]
  6. Chet I., Henis Y. 1967; Hyphal and sclerotial walls of Sclerotium rolfsii: Chemical composition and dissolution by lytic enzymes. The First Israel Congress of Plant Pathology31
    [Google Scholar]
  7. Chet I., Henis Y. 1969; Effect of catechol and disodium-EDTA on melanin content of hyphal and sclerotial walls of Sclerotium rolfsii Sacc. and the role of melanin in the susceptibility of these walls to β-(1→3) glucanase and chitinase. Soil Biol. & Biochem1 in the Press
    [Google Scholar]
  8. Chet I., Henis Y., Mitchell R. 1967; Chemical composition of hyphal and sclerotial walls of Sclerotium rolfsii Sacc. Can. J. Microbiol 13:37
    [Google Scholar]
  9. Edwards G. A., Edwards M. R. 1960; The intracellular membranes of Blastomyces dermatitidis. Am. J. Bot 47:622
    [Google Scholar]
  10. Gay J. L., Greenwood A. D. 1966; Structural aspects of zoospore production in Saprolegnia ferax with particular reference to the cell and vacuolar membranes. In The Fungus Spore, Proceedings of the 18th Symposium of the Colston Research SocietyBristol95
    [Google Scholar]
  11. Icochea T. 1966; Comparative studies with Sclerotium coffeicolum and S. rolfsii. Ph.D. Dissertation North Carolina State University Aycock R. Stem rot and other diseases caused by S. rolfsii. Tech. Bull174 N. Carol, agric. Exp. Stn Raleigh, N.C:
    [Google Scholar]
  12. Joham H. E. 1943; A nutritional study of the fungus Sclerotium rolfsii. M.Sc. thesis The Texas Agricultural and Mechanical University;
    [Google Scholar]
  13. Nadakavukaren M. J. 1963; Fine structure of microsclerotia of Verticillium albo-atrum, Reinke & Berth. Can. J. Microbiol 9:411
    [Google Scholar]
  14. Pease D. C. 1964 Histological Techniques for Electron Microscopy New York: Academic Press;
    [Google Scholar]
  15. Schmidt R., Liese W. 1968; Structural and histochemical observations on fungal fibre hyphae. In Electron Microscopy Bocciarelli D. S. 2415 Roma; Tipografia Poliglotta Vaticana:
    [Google Scholar]
  16. Scurti J. C., Converso L. 1965; Sulla struttura microscopica e ultromicroscopica degli sclerozi di Typhula sp. Caryologia 18:263
    [Google Scholar]
  17. Townsend B. B., Willetts H. J. 1954; The development of sclerotia of certain fungi. Trans. Br. mycol. Soc 37:213
    [Google Scholar]
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