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Volume 57, Issue 3

The Metabolism of Starch, Maltose, Glucose and Some Other Sugars by the Rumen Ciliate Free

Abstract

Summary

Washed suspensions of grown and incubated anaerobically incorporated C from [C]glucose into the cell pool as [C]glucose, maltose and hexose phosphate and into protozoal polysaccharide and intracellular bacteria as a glucose polymer. The uptake of sugars into the pool was by an active process (predominant at low sugar concentrations) probably into the protozoal endoplasm, and by a passive process (predominant at high concentrations) into another part of the cell. Uptake of glucose by the active process was not sufficiently rapid to increase the level of glucose in the pool by more than 17% per hour; this was probably insufficient to increase appreciably the rate of glucose utilization. These protozoa hydrolysed starch to maltose and glucose and the level of these sugars in the pool of protozoa metabolizing starch grains was controlled in part by the inhibitory effect of these sugars on the enzymes that formed them. Studies on the enzymes involved in the metabolism of starch showed that the glucose in starch was hydrolysed to free glucose before phosphorylation. In experiments on the effect of carbohydrates on the survival of protozoa, starch was the most effective in prolonging protozoal life, followed by maltose and glucose in that order. The greater effectiveness of maltose was correlated with the finding that the rate of uptake of maltose carbon was always greater than that for glucose when the two sugars were present in equimolar concentrations. However, the rate for both sugars was markedly decreased in the presence of penicillin and neomycin. Evidence is presented that the protozoal ectoplasm may be freely permeable to sugars in the medium and that there is a barrier between the ectoplasm and endoplasm. C from [C]starch grains was incorporated by the protozoa but there was no synthesis of protozoal protein from carbohydrate.

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© Society for General Microbiology 1969
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1969-08-01
2024-04-27
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References

  1. Abou Akkada A. R., Howard B. H. 1960; The biochemistry of rumen protozoa. 3. The carbohydrate metabolism of entodinium. Biochem. J 76:445
     [Google Scholar]
  2. Agarwal D. P., Sanwal G. G., Krishnan P. S. 1963; Improved paper chromatographic separation of sugar phosphates using borate impregnated paper. Analyst, Lond 88:969
     [Google Scholar]
  3. Bandurski R. S., Axelrod B. 1951; The chromatographic identification of some biologically important phosphate esters. J. biol. Chem 185:657
     [Google Scholar]
  4. Bryant M. P., Robinson I. M. 1963; Apparent incorporation of ammonia and amino acid carbon during growth of selected species of ruminal bacteria. J. Dairy Sci 46:150
     [Google Scholar]
  5. Coleman G. S. 1958; Maintenance of oligotrich protozoa from the sheep rumen in vitro. Nature, Lond 182:1104
     [Google Scholar]
  6. Coleman G. S. 1960a; The cultivation of sheep rumen oligotrich protozoa in vitro. J. gen. Microbiol 22:555
     [Google Scholar]
  7. Coleman G. S. 1960b; Effect of penicillin on the maintenance of rumen oligotrich protozoa. Nature Lond 187:518
     [Google Scholar]
  8. Coleman G. S. 1962; The preparation and survival of almost bacteria free suspensions of Entodinium caudatum. J. gen. Microbiol 28:271
     [Google Scholar]
  9. Coleman G. S. 1964a; The metabolism of 14C-glycine and 14C-bicarbonate by washed suspensions of the rumen ciliate Entodinium caudatum. J. gen. Microbiol 35:91
     [Google Scholar]
  10. Coleman G. S. 1964b; The metabolism of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum. J. gen. Microbiol 37:209
     [Google Scholar]
  11. Coleman G. S. 1967a; The metabolism of free amino acids by washed suspensions of the rumen ciliate Entodinium caudatum. J. gen. Microbiol 47:433
     [Google Scholar]
  12. Coleman G. S. 1967b; The metabolism of the amino acids of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum. J. gen. Microbiol 47:449
     [Google Scholar]
  13. Coleman G. S., Hall F. J. 1966; Bacterial feeding by the rumen ciliate Entodinium caudatum. J. gen. Microbiol 44:iii
     [Google Scholar]
  14. Dixon M., Webb E. C. 1964 Enzymes London: Longmans Green and Co., Ltd;
     [Google Scholar]
  15. Gutllaume J., Osteux R. 1955; Détermination des acides aliphatiques volatils de C1 à C6 dansles liquids biologiques par chromatographie du papier. C. r. hebd. Seanc. Acad. Sci. Paris: 241:501
     [Google Scholar]
  16. Holter H. 1965; Passage of particles and macromolecules through cell membranes. Symp. Soc. gen.Microbiol 15:89
     [Google Scholar]
  17. Horrocks R. H., Manning G. B. 1949; Partition chromatography on paper. Identification of reducing substances in urine Lancet: i:1042
     [Google Scholar]
  18. Huggett A., St G., Nixon D. A. 1957; Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet: ii:368
     [Google Scholar]
  19. Leloir L. F., Cardini C. E. 1957; Characterization of phosphorus compounds by acid lability. In Methods of Enzymology Colowick S. P., Kaplan N. O. 3:840
     [Google Scholar]
  20. Oxford A. E. 1951; The conversion of certain soluble sugars to a glucosan by holotrich ciliates inthe rumen of sheep. J. gen. Microbiol 5:83
     [Google Scholar]
  21. Park J. T., Johnson M. J. 1949; A submicrodetermination of glucose. J. biol. Chem 181:149
     [Google Scholar]
  22. Potter V. R., Elvehjem C. A. 1936; A modified method for the study of tissue oxidations. J. biol. Chem 114:495
     [Google Scholar]
  23. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies on biosynthesis in Escherichia coli. Publs.Carnegie Instn No607
     [Google Scholar]
  24. Smith I. 1960 Chromatographie and Electrophoretic Techniques London: William Heinemann Medical Books, Ltd;
     [Google Scholar]
  25. Sugden B. 1953; The cultivation and metabolism of oligotrich protozoa from the sheep’s rumen. J. gen. Microbiol 9:44
     [Google Scholar]
  26. White R. W. 1966; Bacterial population of the rumen ciliate Entodinium caudatum cultured in vitro. J. gen. Microbiol 42:xii
     [Google Scholar]
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The Metabolism of Starch, Maltose, Glucose and Some Other Sugars by the Rumen Ciliate Entodinium Caudatum
Microbiology 57, 303 (1969); https://doi.org/10.1099/00221287-57-3-303
/content/journal/micro/10.1099/00221287-57-3-303
/content/journal/micro/10.1099/00221287-57-3-303
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