1887

Abstract

SUMMARY

Two methods were developed to demonstrate a small fraction (10) of phage-sensitive cells in a phage-resistant population with a high degree of accuracy, using the plexiglass phage titration method (Horváth & Alföldi, 1954).

Phage-sensitivity was transferable by DNA isolated from 168 (SPO-1 phage-sensitive) to the recipient strain of 168 (SPO-1 phage-resistant) in transformation experiments. The number of 168 transformants was a function of the concentration of the transforming DNA. The and characters are not linked. The competence curves for the number of 168 and 168 transformants were similar. The phenotypic lag was found to be 5 hr 30 min. The maximum frequency of 168 cells among the transformants was 2 to 5 %.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-58-1-69
1969-09-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/58/1/mic-58-1-69.html?itemId=/content/journal/micro/10.1099/00221287-58-1-69&mimeType=html&fmt=ahah

References

  1. Goodgal S. H. 1961; Studies on transformation of Hemophilus influenzae IV. Linked and unlinked transformations. J. gen. Physiol 45:205
    [Google Scholar]
  2. Horváth S. 1967; Development of competence in cultures of Bacillus subtilis inoculated with different numbers of bacteria. J. gen. Microbiol 48:215
    [Google Scholar]
  3. Horváth S. 1968; Competence in Bacillus subtilis transformation system. J. gen. Microbiol 51:85
    [Google Scholar]
  4. Horváth S. 1969; Transformation of phage-resistance in Bacillus subtilis . Experientia 25:312
    [Google Scholar]
  5. Horváth S., Alföldi L. 1954; A new and sensitive method of phage titration on plastic trays. Acta microbiol. hung 1:495
    [Google Scholar]
  6. Kelly M. S. 1967; The causes of instability of linkage in transformation of Bacillus subtilis . Molec. gen. Genetics 99:350
    [Google Scholar]
  7. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact 63:399
    [Google Scholar]
  8. Luria S. R., Delbrück M. 1943; Mutations of bacteria from virus sensitivity to virus resistance. Genetics, Princeton 28:491
    [Google Scholar]
  9. Michel J. F., Sicard A. M., Ephrussi-Taylor E. 1964; Comparaison de deux methodes d’études de liasons faibles dans la transformation du pneumocoque. Expl Cell Res 36:368
    [Google Scholar]
  10. Ravin A. W. 1961; The genetics of transformation. Adv. Genet 10:61
    [Google Scholar]
  11. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. biophys. Acta 72:619
    [Google Scholar]
  12. Spizizen J. 1958; Transformation of a biochemically deficient strain of Bacillus subtilis by deoxvribonucleate. Proc. natn. Acad. SciU.S.A. 441072
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-58-1-69
Loading
/content/journal/micro/10.1099/00221287-58-1-69
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error