1887

Abstract

SUMMARY: Isolated bacterial wall material from four Gram-positive and four Gram-negative organisms bound crystal violet as well as or better than intact bacteria. Dye binding ability varied between species. There was no correlation between the dye binding ability of isolated wall material and the Gram characteristics of the intact bacteria.

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/content/journal/micro/10.1099/00221287-59-2-223
1969-12-01
2024-05-06
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References

  1. Bartholomew J. W. 1962; Variables influencing results, and the precise definition of steps in Gram staining as a means of standardizing the results obtained.. Stain Technol 37:139
    [Google Scholar]
  2. Bartholomew J. W., Cromwell T. 1965; Relative contribution of the cell wall, cytoplasmic membrane, and cytoplasm to the Gram-positive characteristic of Bacillus megaterium. . J. Bact 90:643
    [Google Scholar]
  3. Bartholomew J. W., Finkelstein H. 1954; Crystal violet binding capacity and the Gram reaction of bacterial cells.. J. Bact 67:689
    [Google Scholar]
  4. Bartholomew J. W., Finkelstein H. 1958; Relationship of cell wall staining to Gram differentiation.. J. Bact 75:77
    [Google Scholar]
  5. Bartholomew J. W., Cromwell T., Gan R. 1965; Analysis of the mechanism of Gram differentiation by use of a filter-paper chromatographic technique.. J. Bact 90:766
    [Google Scholar]
  6. Benians T. H. C. 1912; Observations on the Gram-positive and acid-fast properties of bacteria.. J. Path. Bact 17:199
    [Google Scholar]
  7. Bisset K. A., Hale C. H. F. 1953; Complex cellular structure in bacteria.. Expl Cell Res 5:449
    [Google Scholar]
  8. Burke V., Barnes M. W. 1929; The cell wall and the Gram reaction.. J. Bact 18:69
    [Google Scholar]
  9. Finkelstein H., Bartholomew J. W. 1953; Quantitative determination of dye uptake by bacterial cells.. Stain Technol 28:177
    [Google Scholar]
  10. Hale C. M. F., Bisset K. A. 1956; A comparison of the staining reaction of cell walls of Azotobacter chroococcum and those of Gram-positive and Gram-negative bacteria.. J. gen. Microbiol 15:423
    [Google Scholar]
  11. Hardwick W. A., Guirard B., Foster J. W. 1951; Antisporulation factors in complex organic media. II. Saturated fatty acids as antisporulation factors.. J. Bact 61:145
    [Google Scholar]
  12. Knaysi G. 1951 Elements of Bacterial Cytology, 2nd ed.. Ithaca, New York: Comstock Publishing Co. Inc.;
    [Google Scholar]
  13. Lamanna C., Mallette M. F. 1954; The cytological basis for the role of the primary dye in the Gram stain.. J. Bact 68:509
    [Google Scholar]
  14. Salton M. R. J. 1953; Cell structure and enzymic lysis of bacteria.. J. gen. Microbiol 9:512
    [Google Scholar]
  15. Salton M. R. J. 1963; The relationship between the nature of the cell wall and the Gram stain.. J. gen. Microbiol 30:223
    [Google Scholar]
  16. Salton M. R. J., Horne R. W. 1951a; Studies on the bacterial cell wall. I. Electron microspical observations on heated bacteria.. Biochim. biophys. Acta 7:19
    [Google Scholar]
  17. Salton M. R. J., Horne R. W. 1951b; Studies on the bacterial cell wall. II. Methods of preparation and some properties of cell walls.. Biochim. biophys. Acta 7:177
    [Google Scholar]
  18. Scherrer R. 1963; Cell structure and quantitative Gram stain of Bacillus megaterium. . J. gen. Microbiol 31:135
    [Google Scholar]
  19. Smyth R. D., Gershenfeld L. 1960; Iodine131 uptake in Gram staining technique.. Stain Technol 35:237
    [Google Scholar]
  20. Wensick F., Boevé J. J. 1957; Quantitative analysis of the Gram reaction.. J. gen. Microbiol 17:401
    [Google Scholar]
  21. Wistreich G. A. 1969 Studies of bacterial cell wall preparation and dye uptake Ph.D. thesis: University of Southern California, Los Angeles, California.;
    [Google Scholar]
  22. Wistreich G. A., Lechtman M. D., Bartholomew J. W., Bils R. 1968; Disruption of bacterial cells by a synthetic zeolite.. Appl. Microbiol 16:1269
    [Google Scholar]
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