1887

Abstract

SUMMARY: Mycelium of potassium-limited had a lowered potassium content and an increased sodium content compared with mycelium grown in presence of excess potassium and no sodium. Potassium uptake involves the exchange for Na and probably also for H.

Total potassium uptake was decreased to half by a fivefold equivalent excess of rubidium, but was essentially unaffected by a 100-fold equivalent excess of sodium. Calcium and magnesium had intermediate effects. Potassium uptake was inhibited by sodium azide (m) and by dinitrophenol (0.3 m). These depressed the equilibrium potassium level but had a much smaller effect on the time required to reach equilibrium. Loss of potassium by K-loaded mycelium to sodium azide or dinitrophenol solutions or to water was small. Much greater losses occurred to solutions of rubidium but not of sodium.

It is suggested that the equilibrium potassium level is determined only in part by exchange-diffusion at the mycelium surface, and that net potassium uptake is a metabolic process.

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1969-12-01
2024-05-06
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References

  1. Bruno G. A., Christian J. E. 1961; Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting technique.. Analyt. Chem 33:1216
    [Google Scholar]
  2. Budd K. 1969a; The assimilation of bicarbonate by Neocosmospora vasinfecta. . Can. J. Microbiol 15:389
    [Google Scholar]
  3. Budd K. 1969b; Net transport of potassium by nongrowing fungal mycelium.. XI Intern. Botan. Congr. Proceedings (In Press.)
    [Google Scholar]
  4. Budd K., Harley J. L. 1962; The uptake and assimilation of ammonia by Neocosmospora vasin fecta. . New Phytol 61:138
    [Google Scholar]
  5. Conway E. J., Duggan F. 1958; A cation carrier in the yeast cell wall.. Biochem. J 69:265
    [Google Scholar]
  6. Conway E. J., Malley E. 1946; The nature of the cation changes during yeast fermentation, with formation of 0.02 N hydrogen ion.. Biochem. J 40:59
    [Google Scholar]
  7. Conway E. J., Moore P. T. 1954; A sodium-yeast and some of its properties.. Biochem. J 57:523
    [Google Scholar]
  8. Epstein W., Schultz S. G. 1966; Cation transport in Escherichia coli. VI. K exchange.. J. gen. Physiol 49:469
    [Google Scholar]
  9. Galdiero F. 1966; The kinetics of potassium exchange in cells of Staphylococcus aureus. . Biochim. biophys. Acta 126:54
    [Google Scholar]
  10. Horowitz N. H., Beadle G. W. 1943; A microbiological method for the determination of choline by use of a mutant of Neurospora.. J. biol. Chem 150:325
    [Google Scholar]
  11. Jackson P. C., Edwards D. G. 1966; Cation effects on chloride fluxes and accumulation levels in barley roots.. J. gen. Physiol 50:225
    [Google Scholar]
  12. Lester G., Hechter O. 1958; Dissociation of rubidium uptake by Neurospora crassa into entry and binding phases.. Proc. natn. Acad. Sci. U.S.A 44:1141
    [Google Scholar]
  13. Rothstein A. 1959; Role of the cell membrane in the metabolism of inorganic electrolytes by microorganisms.. Bact. Rev 23:175
    [Google Scholar]
  14. Rothstein A. 1965 The Fungi: an Advanced Treatise Ed. by Ainsworth G. C., Sussman A. S. 1429 New York: Academic Press, Inc.;
    [Google Scholar]
  15. Schultz S. G., Epstein W., Solomon A. K. 1963; Cation transport in Escherichia coli. IV. Kinetics of net K uptake.. J. gen. Physiol 47:329
    [Google Scholar]
  16. Slayman C. L. 1965a; Electrical properties of Neurospora crassa. Effects of external cations on the intracellular potential.. J. gen. Physiol 49:69
    [Google Scholar]
  17. Slayman C. L. 1965b; Electrical properties of Neurospora crassa. Respiration and the intracellular potential.. J. gen. Physiol 49:93
    [Google Scholar]
  18. Slayman C. W., Tatum E. L. 1964; Potassium transport in Neurospora. I. Intracellular sodium and potassium concentrations and cation requirements for growth.. Biochim. biophys. Acta 88:578
    [Google Scholar]
  19. Slayman C. W., Tatum E. L. 1965; Potassium transport in Neurospora. II. Measurement of steady-state potassium fluxes.. Biochim. biophys. Acta 102:149
    [Google Scholar]
  20. Ussing H. H. 1949; The distinction by means of tracers between active transport and diffusion.. Acta physiol, scand 19:43
    [Google Scholar]
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