1887

Abstract

SUMMARY:Phenol-water extracted lipopolysaccharides (LPS) from 9343 did not contain heptose or 2-keto-3-deoxy-octonate. The sugar components identified were glucosamine, galactosamine, glucose, galactose, fucose, rhamnose and traces of mannose. An unidentified amino compound was found. The preparations were free of nucleic acids and protein constituted only a minor fraction of the LPS. The LPS preparations had endotoxic effect when tested in rabbits, but the endotoxic potency was low.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-61-1-15
1970-04-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/61/1/mic-61-1-15.html?itemId=/content/journal/micro/10.1099/00221287-61-1-15&mimeType=html&fmt=ahah

References

  1. Adams G. A., Tornabene T. G., Yaguchi M. 1969; Cell wall lipopolysaccharides from Neisseria catarrhalis . Canadian Journal of Microbiology 15:365
    [Google Scholar]
  2. Crumpton M. J., Davies D. A. L. 1958; The isolation of D-fucosamine from the specific polysaccharide of Chromobacterviolaceum(NCTC7917). Biochemical Journal 70:729
    [Google Scholar]
  3. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. Journal of Biological Chemistry 204:983
    [Google Scholar]
  4. Dische Z. 1955; New color reactions for determination of sugars in polysaccharides. In Methods of Biochemical Analysis Glick D. Ed. by 2 p. 313 New York: Interscience Publishers, Inc.;
    [Google Scholar]
  5. Gardell S. 1953; Separation on Dowex 50 ion exchange resin of glucosamine and galactosamine and their quantitative determination. Acta Chemica Scandinavica 7:207
    [Google Scholar]
  6. Hickman J., Ashwell G. 1966; Isolation of a bacterial lipopolysaccharide from Xanthomonascampestris containing 3-acetamido-3.6-dideoxy-D-galactose and d-rhamnose. Journal of Biological Chemistry 241:1424
    [Google Scholar]
  7. Hofstad T. 1968; Chemical characteristics of Bacteroidesmelaninogenicus endotoxin. Archives of Oral Biology 13:1149
    [Google Scholar]
  8. Kabat E. A., Mayer M. M. 1961 Experimental Immunochemistry, 2nd ed.. p. 476 Springfield: Charles C.Thomas;
    [Google Scholar]
  9. Kasai N., Nowotny A. 1967; Endotoxicglyco-lipid from a heptoseless mutant of Salmonella minnesota . Journal of Bacteriology 94:1824
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  11. Lüderitz O., Staub A. M., Westphal O. 1966; Immunochemistry of o and R antigens of Salmonella and related Enterobacteriaceae. Bacteriological Reviews 30:192
    [Google Scholar]
  12. Osborn M. J. 1963; Studies on the Gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxy-octonate in the lipopolysaccharide of Salmonella typhimurium . Proceedings of the National Academy of Sciences of the United States of America 50:499
    [Google Scholar]
  13. Partridge S. M. 1948; Filter paper chromatography of sugars. I. General description and application of the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. Biochemical Journal 42:238
    [Google Scholar]
  14. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochemical Journal 61:586
    [Google Scholar]
  15. Sharon N., Jeanloz R. W. 1960; The diaminohexose component of a polysaccharide isolated from Bacillus subtilis . Journal of Biological Chemistry 235:1
    [Google Scholar]
  16. Sharon N., Shif I., Zehavi U. 1964; The isolation of D-fucosamine (2-amino-2.6-dideoxy-d-galactose) from polysaccharides of Bacillus. Biochemical Journal 93:210
    [Google Scholar]
  17. Snyder F., Stephens N. 1959; A simplified spectrophotometric determination of ester groups in lipids. Biochimica et Biophysica Acta 34:244
    [Google Scholar]
  18. Volk W. A. 1966; Cell wall lipopolysaccharides from Xanthomonas species. Journal of Bacteriology 91:39
    [Google Scholar]
  19. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coliB. I. Identification. Journal of Biological Chemistry 234:705
    [Google Scholar]
  20. Westphal O., Lüderitz O., Bister F. 1952; Über die Extraktion von Bakterienmit Phenol/ Wasser. Zeitschrift für Naturforschung 7B:148
    [Google Scholar]
  21. Winzler R. J. 1955; Determination of serum glyco-proteins. In Methods of Biochemical Analysis Glick D. Ed. by 2 p. 279 New York: Interscience Publishers, Inc;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-61-1-15
Loading
/content/journal/micro/10.1099/00221287-61-1-15
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error