1887

Abstract

SUMMARY: Subcellular fractionation of the ciliate protozoon has been carried out using sucrose density gradients in zonal rotors. Fractions containing mitochondria, peroxisomes, ‘microsomes’ and organelles containing acid hydrolases have been characterized. Specific activities of acid phosphatase, DNase and -acetyl glucosaminidase in whole homogenates increase during the growth cycle, while those of mitochondrial enzyme systems (succinoxidase and 2-oxoglutarate oxidase) as well as those of NADH- and NADPH-cytochrome oxidoreductases decrease. Increased sedimentability of DNase and -acetylglucosaminidase is manifest in homogenates of cells grown for 6 days but the density distributions of the sedimentable portions of these enzymes and also that of acid phosphatase is notaltered. Marked changes in density distributions of lysosomal enzymes occur on starvation. Resolution of the heterogeneous lysosomal population into several discrete populations with different enzyme complements has been achieved. No significant alteration of mitochondrial density occurs in the stationary phase of growth or on starvation, whereas the median buoyant density of peroxisomes is increased under both these conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-65-2-209
1971-02-01
2024-04-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/65/2/mic-65-2-209.html?itemId=/content/journal/micro/10.1099/00221287-65-2-209&mimeType=html&fmt=ahah

References

  1. Anderson N. G., Barringer H. P., Cho N., Nunley C. E., Babelay E. F., Canning R. E., Rankin C. T. Jun. 1966; The development of low-speed ‘A’series zonal rotors.. National Cancer Monograph 21:113–136
    [Google Scholar]
  2. Anderson N. G., Waters D. A., Fisher W. D., Cline G. B., Nunley C. E., Elrod L. H., Rankin C. T. 1967; Analytical techniques for cell fractions. V. Characteristic of the BXIV and BXV zonal centrifuge rotors.. Analytical Biochemistry 21:235–252
    [Google Scholar]
  3. Baudhuin P., ller M., Poole B., deDuve C. 1965; Non-mitochondrial oxidizing particles (microbodies) in rat liver and in Tetrahymena pyriformis. . Biochemical and Biophysical Research Communications 20:53–59
    [Google Scholar]
  4. Borroah J. K., Leaback D. H., Walker P. G. 1961; Studies on glucosaminidase. II. Substrates for N-acetyl β-glucosaminidase.. Biochemical Journal 78:105–110
    [Google Scholar]
  5. Brightwell R., Lloyd D., Turner G., Venables S. E. 1968; Subcellular fractionation of Tetrahymena pyriformis by zonal centrifugation: changes in enzyme distribution during the growth cycle.. Biochemical Journal 109:42
    [Google Scholar]
  6. Chance B., Williams G. R. 1955; Respiratory enzymes in oxidative phosphorylation.. Journal of Biological Chemistry 217:383–397
    [Google Scholar]
  7. De Duve C. 1967; General principles.. Enzyme Cytology1 Edited by Roodyn D. B. New York: Academic Press.;
    [Google Scholar]
  8. De Duve C., Berthet J., Beaufay H. 1959; Gradient centrifugation of cell particles. Theory and applications.. Progress in Biophysical Chemistry 9:326–369
    [Google Scholar]
  9. Eichel H. J. 1954; Studies on the oxidation of succinic acid by cell-free homogenates of Tetrahymena pyriformis s and w.. Journal of Biological Chemistry 206:159–167
    [Google Scholar]
  10. Eichel H. J. 1960; Electron transport and phosphorylation inhibitor in Tetrahymena and evidence for its formation by a phospholipase.. Biochimica et biophysica acta 43:364–370
    [Google Scholar]
  11. Elliott A. M., Bak I. J. 1964; The fate of mitochondria during ageing in Tetrahymena pyriformis. . Journal of Cell Biology 20:113–129
    [Google Scholar]
  12. Hamburger K., Zeuthen E. 1957; Synchronous divisions in Tetrahymena pyriformis as studied in an inorganic medium.. Experimental Cell Research 13:443–451
    [Google Scholar]
  13. Hughes D. E., Lloyd D., Brightwell R. 1970; Structure, function and distribution of organelles in prokaryotic and eukaryotic microbes.. Symposia of the Society for General Microbiology 20:295–322
    [Google Scholar]
  14. Kobayshi S. 1965; Preparation and properties of mitochondria from the ciliated protozoon Tetrahymena.. Journal of Biochemistry (Tokio) 58:444–457
    [Google Scholar]
  15. Lazarus L. H., Scherbaum O. H. 1961; Activity of ribonuclease, acid phosphatase and phosphodiesterase in Tetrahymena pyriformis during growth.. Journal of Cell Biology 36:415–418
    [Google Scholar]
  16. Levy M. R., Elliott A. M. 1968; Biochemical and ultrastructure changes in Tetrahymena pyriformis during starvation.. Journal of Protozoology 15:208–222
    [Google Scholar]
  17. Lloyd D., Brookman J. S. G. 1967; An oxygen electrode reaction vessel.. Biotechnology and Bioengineering 9:271–272
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Lück H. 1963; Catalase.. Methods of Enzymatic Analysis885 Edited by Bergmeyer H. U. New York: Academic Press.;
    [Google Scholar]
  20. Mahler H. R. 1955; DPNH cytochrome c reductase (animal).. Methods in Enzymology 2:688–693
    [Google Scholar]
  21. Müller M., Baudhuin P., deDuve C. 1966; Lysosomes in Tetrahymena pyriformis. . Journal of Cellular Physiology 68:165–175
    [Google Scholar]
  22. Müller M., Hogg J. F., deDuve C. 1968; Distribution of tricarboxylic acid cycle and of gloxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis. . Journal of Biological Chemistry 243:5385–5395
    [Google Scholar]
  23. Parsons D. F. 1966; Ultrastructure and molecular aspects of cell membranes.. Proceedings of the Seventh Canadian Cancer Conference193 Oxford: Pergamon Press.;
    [Google Scholar]
  24. Thompson G. A. Jun. 1967; Studies of membrane formation in Tetrahymena pyriformis. (a) Rate of phospholipid biosynthesis.. Biochemistry 6:2015–2021
    [Google Scholar]
  25. Torriani A. 1960; Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. . Biochimica et biophysica acta 38:460–468
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-65-2-209
Loading
/content/journal/micro/10.1099/00221287-65-2-209
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error