1887

Abstract

Summary: When crude membrane preparations from broth-grown ( 9372) were incubated with 20 p.p.m. technical chlordane inhibition of NADH oxidase reached 45% in only 3 min. and 75% after 1 h. Maximum inhibition was obtained at this concentration of chlordane. NADH: acceptor oxidoreductase (EC 1.6.99.-) of membrane preparations was insensitive to 20 p.p.m. chlordane. Disruption of the remainder of the electron-transport chain appeared to be general though more severe in the region between NADH dehydrogenase and cytochrome . A generalized assay for ATPases revealed no sensitivity to chlordane over a concentration range 20 to 100 p.p.m. and a pre-incubation period of 1 h. The inhibition of TCA cycle enzymes observed, subsequent to the incubation of high-speed supernatant fluid with 20 p.p.m. chlordane, extended the range of chlordane-sensitive phenomena to enzymes other than those that are membrane bound.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-69-1-15
1971-11-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/69/1/mic-69-1-15.html?itemId=/content/journal/micro/10.1099/00221287-69-1-15&mimeType=html&fmt=ahah

References

  1. Anfinsen C. B. 1955; Aconitase from pig heart muscle. In Methods in Enzymology 1 p. 695 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  2. Beechey R. B. 1966; The uncoupling of respiratory chain phosphorylation by 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole. Biochemical Journal 98:284–289
    [Google Scholar]
  3. Bonner W. 1955; Succinic dehydrogenase. In Methods in Enzymology 1 p. 723 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  4. Brodie A. F. 1955; DPNH cytochrome c reductase (bacterial). In Methods in Enzymology II p. 693 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  5. Buchel K. H., Korte F., Beechey R. B. 1965; Uncoupling of the oxidative phosphorylation in bacteria by NH-acidic benzimidazoles. Angewandte Chemie International Edition 4:788–789
    [Google Scholar]
  6. Colvin H. J., Phillips A. T. 1968; Inhibition of electron transport and cholinesterases by endrin. Bulletin of Environmental Contamination and Toxicology 3:106–115
    [Google Scholar]
  7. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Biological Chemistry 66:375
    [Google Scholar]
  8. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum protein by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  9. Hall D. O., Palmer J. M. 1968; Mitochondrial research today. Nature; London: 221717–723
    [Google Scholar]
  10. Harold F. M., Baarda J. R., Baron C., Abrams A. 1969; Inhibition of membrane-bound adenosine triphosphatase and of cation transport in Streptococcus faecalis by N, N′-dicyclohexylcarbodimide. Journal of Biological Chemistry 244:2261–2268
    [Google Scholar]
  11. Jacobs E. E. 1960; Phosphorylation coupled to electron transport initiated by substituted phenylenedia-mines. Biochemical and Biophysical Research Communications 3:536–539
    [Google Scholar]
  12. Johnston C. D. 1951; The in vitro effect of DDT and related compounds on the succinoxidase system of rat heart. Archives of Biochemistry and Biophysics 31:375–382
    [Google Scholar]
  13. Kashket E. R., Brodie A. F. 1962; Effects of near-ultraviolet irradiation on growth and oxidative metabolism of bacteria. Journal of Bacteriology 83:1094–1100
    [Google Scholar]
  14. Kaufman S. 1955; α-Ketoglutaric dehydrogenase system and phosphorylating enzyme from heart muscle. In Methods in Enzymology 1 p. 714 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  15. King T. E., Howard R. L. 1967; Preparations and properties of soluble NADH dehydrogenases from cardiac muscle. In Methods in Enzymology x p. 275 Estabrook R. W., Pullman M. E. Edited by London: Academic Press;
    [Google Scholar]
  16. Koch R. B. 1969a; Chlorinated hydrocarbon insecticides: inhibition of rabbit brain ATPase activities. Journal of Neuro-chemistry 16:269–271
    [Google Scholar]
  17. Koch R. B. 1969b; Inhibition of animal tissue ATPase activities by chlorinated hydrocarbon insecticides. Chemical Biological Interactions 1:199–209
    [Google Scholar]
  18. Koch R. B., Cutkomp L. K., Do F. M. 1969; Chlorinated hydrocarbon insecticide inhibition of cockroach and honey bee ATPases. Life Sciences 8:289–297
    [Google Scholar]
  19. Massey V. 1955; Fumerase. In Methods in Enzymology 1 p. 730 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  20. Morrison P. E., Brown A. W. A. 1954; The effects of insecticides on cytochrome oxidase obtained from the American cockroach. Journal of Economic Entomology 47:723–730
    [Google Scholar]
  21. Nelson B. D., Williams C. 1971; Action of cyclodiene pesticides on oxidative metabolism in the yeast Saccharomyces cerevisiae . Journal of Agricultural and Food Chemistry 19:339–341
    [Google Scholar]
  22. Ochoa S. 1955a; Crystalline condensing enzyme from pig heart. In Methods in Enzymology 1 p. 687 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  23. Ochoa S. 1955b; Isocitric dehydrogenase system (TPN) from pig heart. In Methods in Enzymology 1 p. 699 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  24. Ochoa S. 1955c; Malic dehydrogenase from pig heart. In Methods in Enzymology 1 p. 736 Colowick S. P., Kaplan N. O. Edited by London: Academic Press;
    [Google Scholar]
  25. Op Den Kamp J. A. F., van Deenen L. L. M., Tomasi V. 1969 In Structure and Functional Aspects of Lipoproteins in Living Systems p. 227 Tria E., Scanu A. M. Edited by London: Academic Press;
    [Google Scholar]
  26. Packer L., Jacobs E. E. 1962; Coupling of phosphorylation to terminal segments of the mitochondrial respiratory chain. Biochemica et biophysica acta 57:371–373
    [Google Scholar]
  27. Patil K. C., Matsumura F., Bratkowski T. A. 1969; Brain ATPases of the resistant and susceptible German cockroach. Journal of Economic Entomology 62:1502–1503
    [Google Scholar]
  28. Ryter A. 1968; Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriological Reviews 32:39–54
    [Google Scholar]
  29. Saklin J. A., Terriere L. C., Remmert L. F. 1955; Effect of DDT on enzymatic oxidation and phosphorylation. Science; New York: 122377–378
    [Google Scholar]
  30. Stoeckenius W., Engelman D. M. 1969; Current models for the structure of biological membranes. Journal of Cell Biology 42:613–646
    [Google Scholar]
  31. Trudgill P. W., Widdus R. 1970; Effects of chlorinated insecticides on metabolic processes in bacteria. Biochemical Journal 118:48–49P
    [Google Scholar]
  32. Trudgill P. W., Widdus R., Rees J. S. 1971; Effects of organochlorine insectides on bacterial growth, respiration and viability. Journal of General Microbiology 69:1–13
    [Google Scholar]
  33. Weinbach E. G., Garbus J. 1969; Mechanism of action of reagents that uncouple oxidative phosphorylation. Nature; London: 2211016–1018
    [Google Scholar]
  34. Wineley C. L., San Clemente C. L. 1970; Effects of pesticides on nitrite oxidation by Nitrobacter agilis . Applied Microbiology 19:214–219
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-69-1-15
Loading
/content/journal/micro/10.1099/00221287-69-1-15
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error