1887

Abstract

SUMMARY: Optimum conditions for acetate oxidation by acetate-adapted cells of and experiments dealing with the inhibition of this oxidation are described. Quantitative relationships in isotope experiments between precursor and product implicate at least succinate, fumarate and malate in the mechanism of acetate oxidation by this organism. The involvement of additional compounds in the oxidation of acetic acid is not excluded by these experiments. However, compounds more oxidized than acetate, such as glycollate and glyoxylic acid, are shown not to be involved in the oxidation of the C-fatty acid.

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/content/journal/micro/10.1099/00221287-7-3-4-221
1952-11-01
2024-05-05
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References

  1. Ajl S. J. 1950; Acetic acid oxidation by Escherichia coli and Aerobacter aerogenes. J. Bact 59:499
    [Google Scholar]
  2. Ajl S. J. 1951a; Studies on the mechanism of acetate oxidation by bacteria. V. Evidence for the participation of fumarate, malate, and oxalacetate in the oxidation of acetic acid by Escherichia coli. J. gen. Physiol 34:785
    [Google Scholar]
  3. Ajl S. J. 1951b; Terminal respiratory mechanisms in microorganisms. Bact. Rev 15:21
    [Google Scholar]
  4. Ajl S. J., Kamen M. D. 1950; Studies on the mechanism of acetate-oxidation by bacteria. Fed. Proc 9:143
    [Google Scholar]
  5. Ajl S. J., Kamen M. D. 1951a; Studies on the mechanism of acetate-oxidation by Escherichia coli. J. biol. Chem 189:845
    [Google Scholar]
  6. Ajl S. J., Kamen M. D. 1951b; A simple and rapid biosynthesis of isotopically labelled succinic acid. J. Amer. chem. Soc 75:2349
    [Google Scholar]
  7. Barron E. S. G., Ardeo M. S., Hearon J. 1950; The mechanism of acetate oxidation by Corynebacterium creatinovorans. Arch. Biochem 29:130
    [Google Scholar]
  8. Dagley S., Dawes E. A., Morrison G. S. 1951; The kinetics of pyruvate production by Aerobacter aerogenes. J. gen. Microbiol 5:508
    [Google Scholar]
  9. Hersey D. F., Ajl S. J. 1951; Phosphorylation due to the oxidation of succinic acid by cell-free extracts of Escherichia coli. J. gen. Physiol 34:295
    [Google Scholar]
  10. LePage G. A. 1950; Measurements of keto acids in normal and neoplastic rat tissue. Cancer Res 10:393
    [Google Scholar]
  11. Lugg J. W. H., Overell B. T. 1948; One and two-dimensional partition chromatographic separations of organic acids on an inert sheet support. Aust. J. sci. Res. A 1:98
    [Google Scholar]
  12. Slade H. D., Werkman C. H. 1943; Assimilation of acetic and succinic acids containing heavy carbon by Aerobacter indologenes. Arch. Biochem 2:97
    [Google Scholar]
  13. Swim H. E., Krampitz L. O. 1950; Evidence for the condensation of acetic acid to succinic acid in Escherichia coli. Bact. Proc125
    [Google Scholar]
  14. Tolbert N. E., Clagett C. O., Burris R. H. 1949; Products of the oxidation of glycolic acid and lactic acid by enzymes from tobacco leaves. >J. biol. Chem 181:905
    [Google Scholar]
  15. Zilversmit D. B., Entenman C., Fishler M. C. 1943; On the calculation of ‘turnover time’ and ‘turnover rate’ from experiments involving the use of labeling agents. J. gen. Physiol 26:325
    [Google Scholar]
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