1887

Abstract

Summary: Mutants blocked in different stages of flagellar development were isolated and examined by light and electron microscopy. Flagellum-less mutants had normal basal bodies but lacked the flagellar shaft and showed abnormalities in the transitional region. Stumpy mutants had very short flagellar shafts in which electrondense material, sometimes arranged in sheet-like arrays, accumulated in the space between the outer tubules and the distended flagellar membrane. Short-flagellum mutants also showed abnormalities in the flagellar shaft; abnormally spaced radial spokes were seen in negatively stained frayed flagella of one of these mutants. The flagellum lengths andflagellar regeneration characteristics of two long-flagellum mutants with defective length control were determined; length was highly variable with some abnormally long flagella, and regeneration was slow and asynchronous.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-3-525
1972-08-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/3/mic-71-3-525.html?itemId=/content/journal/micro/10.1099/00221287-71-3-525&mimeType=html&fmt=ahah

References

  1. DIPPELL R. V. (1968); The development of basal bodies in Paramaecium.. Proceedings of the National Academy of Sciences of the United Statesof America 61:461–468
    [Google Scholar]
  2. EBERSOLD W. T., LEVINE R. P., LEVINE E. E., OLMSTED M. A. (1962); Linkage maps in Chlamydomonas reinhardii.. Genetics 47:531–543
    [Google Scholar]
  3. HOPKINS J. M. (1970); Subsidiary components of the flagella of Chlamydomonas reinhardii.. Journal of Cell Science 7:823–839
    [Google Scholar]
  4. Jacobs M., McVittie A. (1970); Identification of the flagellar proteins of Chlamydomonas reinhardii.. Experimental Cell Research 63:53–61
    [Google Scholar]
  5. JOHNSON U. G., PORTER K. R. (1968); Fine structure of cell division in Chlamydomonas reinhardii.. Journal of Cell Biology 38:403–405
    [Google Scholar]
  6. LAEMMLI U. K., BEGUIN F., GUJER-KELLENBERGER G. (1970); A factor preventing the major head protein of bacteriophage T4 from random aggregation.. Journal of Molecular Biology 47:69–85
    [Google Scholar]
  7. LUFT J. H. (1961); Improvements in epoxy resin embedding methods.. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  8. MCVITTIE A. (1969); Studies on flagella-less, stumpy and short-flagellum mutants of Chlamydomonas reinhardii.. Proceedings of the Royal Society B 173:59–60
    [Google Scholar]
  9. MCVITTIE A. (1972); Genetic studies on flagellum mutants of Chlamydomonas reinhardii.. Genetical Research (In the press.)
    [Google Scholar]
  10. OUTKA D. E., KLUSS B. C. (1967); The amoeba-to-flagellate transformation in Tetramitus rostratus. II.Microtubular morphogenesis.. Journal of Cell Biology 35:323–346
    [Google Scholar]
  11. RANDALL J. T. (1969); The flagellar apparatus as a model organelle for the study of growth and morpho-poiesis.. Proceedings of the Royal Society B 173:31–58
    [Google Scholar]
  12. RANDALL J. T., CAVALIER-SMITH T., MCVITTIE A., WARR J. R., HOPKINS J. M. (1967) Developmental and control processes in the basal bodies and flagella of Chlamydomonas reinhardii.. In Control Mechanisms in Developmental Processes. 26th Symposium ofthe Society for Developmental Biology. New York and London:: Academic Press.;
    [Google Scholar]
  13. RANDALL J. T., WARR J. R., HOPKINS J. M., MCVITTIE A. (1964); A single-gene mutation of Chlamydomonas reinhardii affecting motility: a genetic and electron microscope study.. Nature, London 203:912–914
    [Google Scholar]
  14. RINGO D. L. (1967); Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas.. Journal of Cell Biology 33:543–571
    [Google Scholar]
  15. ROSENBAUM J. L., MOULDER J. E., RINGO D. L. (1969); Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins.. Journal of Cell Biology 41:600–619
    [Google Scholar]
  16. WARR J. R., MCVITTIE A., RANDALL J. T., HOPKINS J. M. (1966); Genetic control of flagellar structure in Chlamydomonas reinhardii.. Genetical Research 7:335–351
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-71-3-525
Loading
/content/journal/micro/10.1099/00221287-71-3-525
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error