1887

Abstract

SUMMARY: Observations on the ultrastructure of protoplasts obtained by treatment of mycelium with RA lytic enzyme show that they were free of wall material. The preparations contained two types of protoplasts, released at different times during the lytic enzyme-mycelium incubation. Those released first contained small vesicles and a high density of ribosomes. The type released later had large vacuoles and fewer ribosomes. It is suggested that these differences reflect the hyphal location and origin of the protoplasts.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-72-3-529
1972-10-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/72/3/mic-72-3-529.html?itemId=/content/journal/micro/10.1099/00221287-72-3-529&mimeType=html&fmt=ahah

References

  1. ARONSON J. M. 1965; The cell wall.. In The Fungi, vol 1:pp. 49–76 Edited by Ainsworth G. C., Sussman A. A. New York: Academic Press;
    [Google Scholar]
  2. BRACKER C. E. 1971; Cytoplasmic vesicles in germinating spores of Gilberteliapersicaria.. Protoplasma 72:381–397
    [Google Scholar]
  3. BULLER A. R. H. 1958; Researches on Fungi,. vol v: ch. n. New York: H66 afner Publishing Co.;
    [Google Scholar]
  4. Campbell C. K. 1970; Fine structure of vegetative hyphae of Aspergillus fumigatus.. Journal of General Microbiology 64:373–376
    [Google Scholar]
  5. Darling S., Theilade J., Birch-Anderson A. 1969; Kinetic and morphological observations on Saccharomyces cerevisiae during spheroplast formation.. Journal of Bacteriology 98:797–810
    [Google Scholar]
  6. GARCIA LOPEZ M. D., RODRIGUEZ AGUIRRE M. J., URUBURU F., VILLANUEVA J. R. 1967; Characterization of the lytic Streptomyces RA as a new strain of Streptomyces venezuelae.. Antonie van Leeuwenhoek 33:315–323
    [Google Scholar]
  7. GIRBARDT M. 1969; Die Ultrastruktur der Apikalregion von Pilzhyphen.. Protoplasma 67:413–441
    [Google Scholar]
  8. Grove S. N., Bracker C. E. 1970; Protoplasmic organization of hyphal tips among fungi: vesicles and spitzenkorper.. Journal of Bacteriology 104:989–1009
    [Google Scholar]
  9. Hawker L. E., Abbott P. McV. 1963; An electron microscope study of maturation and germination of sporangiospores of two species of Rhizopus.. Journal of General Microbiology 32:295–298
    [Google Scholar]
  10. Hawker L. E., Hendy R. J. 1963; An electron microscope study of germination of conidia of Botrytis cinerea.. Journal of General Microbiology 33:43–46
    [Google Scholar]
  11. HAVELKOVA M. 1966; A comparative study of submicroscopic structures of protoplasts of various yeast species.. Folia microbiologica, Praha 11:453–458
    [Google Scholar]
  12. HEATH I. B., GAY J. L., GREENWOOD A. D. 1971; Cell wall formation in the saprolegniales: cytoplasmic vesicles underlying developing walls.. Journal of General Microbiology 65:225–232
    [Google Scholar]
  13. KAWAKAMI N. 1961; Thread-like mitochondria in yeast cells.. Experimental Cell Research 25:179–181
    [Google Scholar]
  14. Kuster E., WILLIAMS S. T. 1964; Selection of media for isolation of Streptomyces.. Nature, London 202:928–929
    [Google Scholar]
  15. MCCLURE W. K., PARK D., ROBINSON P. M. 1968; Apical organisation in the somatic hyphae of fungi.. Journal of General Microbiology 50:177–182
    [Google Scholar]
  16. Manocha M. S. 1968; Electron microscopy of the conidial protoplasts of Neurospora crassa.. Canadian Journal of Botany 46:1561–1564
    [Google Scholar]
  17. MARCHANT R., PEAT A., BANBURY G. H. 1967; The ultrastructural basis of hyphal growth.. New Phytologist 66:623–629
    [Google Scholar]
  18. MOORE R. T., MCALEAR J. H. 1963; Fine structure of mycota: 9. Fungal mitochondria.. Journal of Ultrastructure Research 8:144–153
    [Google Scholar]
  19. NECAS O. 1964; Mechanism of regeneration of yeast protoplasts. III. Electron microscopy of growing protoplasts.. Folia microbiologica, Praha 11:371–377
    [Google Scholar]
  20. PEBERDY J. F., GIBSON R. K. 1971; Regeneration of Aspergillus nidulans protoplasts.. Journal of General Microbiology 69:325–330
    [Google Scholar]
  21. Prusso D. C., Wells K. 1967; Sporobolomyces roseus. I. Ultrastructure.. Mycologia 59:337–348
    [Google Scholar]
  22. REYNOLDS E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.. Journal of Cell Biology 17:208–213
    [Google Scholar]
  23. ROBERTSON N. F. 1965 The mechanism of cellular extension and branching. In The Fungi vol 1 pp. 613–623, Edited by. Ainsworth G. C., Sussman A. A. New York: Academic Press;
    [Google Scholar]
  24. Rouiller C. 1960; Physiological and pathological changes in mitochondrial morphology.. International Review of Cytology 9:227–292
    [Google Scholar]
  25. SAGARA Y. 1969; Studies of protoplasts of Geotrichum candidum: mechanism of formation of protoplasts and their physiological and morphological properties.. Tokushima Journal of Experimental Medicine 16:56–69
    [Google Scholar]
  26. SHATKIN A. J., TATUM E. L. 1959; Electron microscopy of Neurospora crassa mycelia.. Journal of Biophysical and Biochemical Cytology 6:423–426
    [Google Scholar]
  27. STREIBLOVA E. 1968; Surface structure of yeast protoplasts.. Journal of Bacteriology 95:700–707
    [Google Scholar]
  28. TANAKA K., YANAGITA T. 1963; Electron microscopy of ultra-thin sections of Aspergillus niger. I. The fine structure of hyphal cells.. Journal of General and Applied Microbiology 9:101–118
    [Google Scholar]
  29. URUBURU F., ELORZA V., VILLANUEVA J. R. 1968; The fine structure of yeast regenerating protoplasts.. Journal of General Microbiology 51:195–198
    [Google Scholar]
  30. ZALOKAR M. 1959; Growth and differentiation of Neurospora hyphae.. American Journal of Botany 46:602–610
    [Google Scholar]
  31. ZALOKAR M. 1965 Integration of cellular metabolism.. In The Fungi vol 1 pp. 377–426 Edited by Ainsworth G. C., Sussman A. A. New York; Academic Press:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-72-3-529
Loading
/content/journal/micro/10.1099/00221287-72-3-529
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error