1887

Abstract

SUMMARY: The amounts of three catabolite repressible enzymes, alkaline protease, neutral protease and α-amylase, produced by NRRL-B3411 growing in a chemostat, depended on the growth-limiting substrate. Limiting growth with glucose was advantageous for α-amylase synthesis while nitrogen-limited growth was advantageous for synthesis of the two proteases. Under the conditions used, continuous cultures were unsuitable for large-scale production of the three enzymes since spontaneous mutations to less productive strains occurred in the long term.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-73-1-35
1972-11-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/73/1/mic-73-1-35.html?itemId=/content/journal/micro/10.1099/00221287-73-1-35&mimeType=html&fmt=ahah

References

  1. Bates C. J., Pasternak C. A. 1965; Further studies on the regulation of amino sugar metabolism in Bacillus subtilis . Biochemical Journal 96:147–154
    [Google Scholar]
  2. Breed R. S., Murray E. G. D., Smith N. R. 1957 Bergey’s Manual of Determinative Bacteriology,, 7th edn.. Baltimore: Williams and Wilkins;
    [Google Scholar]
  3. Butterworth D. 1967; Factors determining the choice of batch or continuous fermentation. In Biology and the Manufacturing Industries. Edited by Brooks M. New York: Academic Press;
    [Google Scholar]
  4. Chaloupka J. 1969; Dual control of megateriopeptidase synthesis. Annales Institute Pasteur 117:631–636
    [Google Scholar]
  5. Chaney A. L., Marbach E. P. 1962; Modified reagents for determination of urea and ammonia. Clinical Chemistry 8:130–132
    [Google Scholar]
  6. Coleman G. 1967; Studies on the regulation of extracellular enzyme formation by Bacillus subtilis . Journal of General Microbiology 49:421–431
    [Google Scholar]
  7. Conn H. J., Jennison M. W., Weeks O. B. 1957; Routine tests for the identification of bacteria. In Manual of Microbiological Methods pp. 140–168 New York: McGraw–Hill;
    [Google Scholar]
  8. Ellsworth R., Telling R. C., East D. N. 1959; The investment value of continuous culture. Journal of Applied Bacteriology 22:138–152
    [Google Scholar]
  9. Fabian J. 1969; Protease synthesis of Bacillus pumilus grown in continuous culture. In Continuous Cultivation of Microorganisms. Edited by Malek I., Beran K., Fencl Z., Munk V., Ricica J., Smrckova H. New York: Academic Press;
    [Google Scholar]
  10. Freese E. B., Cole R. M., Klofat W., Freese E. 1970; Growth sporulation and enzyme defects of glucosamine mutants of Bacillus subtilis . Journal of Bacteriology 101:1046–1062
    [Google Scholar]
  11. Goldenbaum P. E., Broman R. L., Dobrogosz W. J. 1970; Cyclic -adenosine monophosphate and N-acetylglucosamine-6-phosphate as regulatory signals in catabolic repression in the lac operon in Escherichia coli . Journal of Bacteriology 103:663–670
    [Google Scholar]
  12. Greenstein J. P., Winitz M. 1961 Chemistry of the Amino Acids vol 2 pp. 1299–1317 New York: Wiley;
    [Google Scholar]
  13. Hanson R. S., Blicharska J., Arnaud M., Szulmajster J. 1964; Observations on the regulation of the synthesis of the tricarboxylic acid cycle enzymes in Bacillus subtilis Marburg. Biochemical and Biophysical Research Communications 17:690–695
    [Google Scholar]
  14. Katz J. 1898; Jahrbuch für wissenschaftliche Botanik 31, 599. Cited by K. Daigen & B. Williams 1970. Catabolite repression and other control mechanisms in carbohydrate utilization. Advances in Microbial Physiology 4:251–324
    [Google Scholar]
  15. Keay L. 1971; Microbial proteases. Process Biochemistry 68:17–21
    [Google Scholar]
  16. Keay L., Moser P. W., Wildi B. S. 1970; Proteases of the genus Bacillus. II. Alkaline proteases. Biotechnology and Bioengineering 12:213–249
    [Google Scholar]
  17. Keay L., Wildi B. S. 1970; Proteases of the genus Bacillus. I. Neutral proteases. Biotechnology and Bioengineering 12:179–212
    [Google Scholar]
  18. Kubitschek H. E. 1970; Nuclear selection in filamentous bacteria: Resolution of the Novick-Szilard Paradox. 10th International Congress of Microbiology 1970: Colloquium C 11–3
    [Google Scholar]
  19. Laishley E. J., Bernlohr R. W. 1966; Catabolic repression of several sporulation enzymes during growth of. Bacillus licheniformis Bacteriological Proceedings97
    [Google Scholar]
  20. Laishley E. J., Bernlohr R. W. 1968; Regulation of arginine and proline catabolism in Bacillus licheniformis . Journal of Bacteriology 96:322–329
    [Google Scholar]
  21. Mandelstam J. 1967; End product repression and the regulation of degradative enzymes. In Regulation of Nucleic Acid, and Protein Synthesis pp. 361–356 Edited by Koningsberger V. V., Bosch L. Amsterdam: Elsevier Publishing Co.;
    [Google Scholar]
  22. Moseley M. H., Keay L. 1970; Purification and characterization of the amylase of Bacillus subtilis nrrl-b3411. Biotechnology and Bioengineering 12:251–271
    [Google Scholar]
  23. Moser H. 1958 The Dynamics of Bacterial Populations Maintained in the Chemostat. Publication G 14. Washington D.C: Carnegie Institution;
    [Google Scholar]
  24. Murrell W. G. 1967; The biochemistry of the bacterial endospore. Advances in Microbial Physiology 1:133–251
    [Google Scholar]
  25. Schaeffer P. 1969; Sporulation and the production of antibiotics, exoenzymes and exotoxins. Bacteriological Reviews 33:48–71
    [Google Scholar]
  26. Schaeffer P., Millet S., Aubert J. P. 1965; Catabolite repression of bacterial sporulation. Proceedings of the National Academy of Sciences of the United States of America. 54:704–711
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-73-1-35
Loading
/content/journal/micro/10.1099/00221287-73-1-35
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error