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Volume 76, Issue 1

Numerical Taxonomy and Laboratory Identification of , and Some Related Bacteria Free

Abstract

Summary: A collection of Gram-positive filamentous and/or diphtheroidal organisms was classified by means of numerical phenetic methods. The collection comprised 76 new isolates from dental plaques, upper respiratory tracts and urinary tracts, and 47 reference strains. The organisms were characterized according to 77 different features. The data were subjected to computer analysis in which the similarity coefficient (S) and the simple matching coefficient (S) were calculated and the results of a single-linkage cluster-analysis technique and a minimum spanning tree technique were compared. The two cluster-analysis techniques gave almost identical results, but with the simple matching coefficient a few strains were grouped together on the basis of negative correlations.

Fixed levels of agreement were chosen to define genera and species. With the matching coefficient five major clusters were recognized at a similarity level of 87.5%. These clusters correspond to the genera and The genera and each contained a number of species. At an agreement level of 90% the clusters of and were identical to those at 87.5% which means that these genera each contain one species. The reference strains labelled and agreed on the 92.5% level which means that they form a single species. A ‘calculated median organism’ for each of these taxo-species is described. A serological grouping by immunofluorescence of all the organisms studied, by means of type-specific antisera to and , corresponded to the numerical grouping.

Characters, both constant and discriminative, were selected to form a set of tests useful for clinical laboratory identification of this group of bacteria.

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© Society for General Microbiology, 1973
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1973-05-01
2024-05-10
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References

  1. Barksdale L. 1970; Corynebacterium diphtheriae and its relatives. Bacteriological Review 34:378–422
     [Google Scholar]
  2. Beers R. J., Lockhart W. R. 1962; Experimental methods in computer taxonomy. Journal of General Microbiology 28:633–640
     [Google Scholar]
  3. Bergan T. 1971; Survey of numerical techniques for grouping. Bacteriological Review 35:379–389
     [Google Scholar]
  4. Bowen W. H. B. 1959 Oral Actinomyces and Their Possible Role in Calculus Formation Thesis. University of Rochester; Rochester, New York:
     [Google Scholar]
  5. Brown J. M., Georg L. K., Waters L. C. 1969; Laboratory identification of Rothia dentocariosa and its occurrence in human clinical materials. Applied Microbiology 17:150–156
     [Google Scholar]
  6. Bousfield I. J. 1972; A taxonomic study of some coryneform bacteria. Journal of General Microbiology 71:441–455
     [Google Scholar]
  7. Buchanan B. B., Pine L. 1962; Characterization of a propionic acid producing actinomycete, Actinomyces propionicus, sp. nov.. Journal of General Microbiology 28:305–323
     [Google Scholar]
  8. Christensen W. B. 1946; Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. Journal of Bacteriology 52:461–466
     [Google Scholar]
  9. Coleman R. M., George L. K. 1969; Comparative pathogenicity of Actinomyces naeslundii and Actionomyces israelii . Applied Microbiology 18:427–432
     [Google Scholar]
  10. Coleman R. M., Georg L. K., Rozzell A. R. 1969; Actinomyces naeslundii as an agent of human actinomycosis. Applied Microbiology 18:420–426
     [Google Scholar]
  11. Cowan S. T., Steel K. J. 1966 Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press;
     [Google Scholar]
  12. Davis G. H. G., Baird-Parker A. C. 1959; The bacterial elements of materia alba. British Dental Journal 106:142–146
     [Google Scholar]
  13. Davis G. H. G., Freer J. H. 1960; Studies upon an oral aerobic actinomycete. Journal of General Microbiology 23:163–178
     [Google Scholar]
  14. Davis G. H. G., Newton K. G. 1969; Numerical taxonomy of some named coryneform bacteria. Journal of General Microbiology 56:195–214
     [Google Scholar]
  15. Gerencser M. A., Slack J. M. 1967; Isolation and characterization of Actinomyces propionicus . Journal of Bacteriology 94:109–115
     [Google Scholar]
  16. Gerencser M. A., Slack J. M. 1969; Identification of human strains of Actinomyces viscosus . Applied Microbiology 18:80–87
     [Google Scholar]
  17. Georg K. L. 1970; Diagnostic procedures for the isolation and identification of the etiologic agents of actinomycosis. In Proceedings International Symposium on Mycoses pp 71–80 Washington, D.C.: World Health Organization;
     [Google Scholar]
  18. Georg L. K., Brown J. M. 1967; Rothia, gen. nov., an aerobic genus of the family Actinomycetaceae . International Journal of Systematic Bacteriology 17:79–88
     [Google Scholar]
  19. Georg L. K., Coleman R. M. 1970; Comparative pathogenicity of various Actinomyces species. In The Actinomycetales pp 35–45 Edited by Prauser H. Jena: Gustav Fischer;
     [Google Scholar]
  20. Georg L. K., Pine L., Gerencser M. A. 1969; Actinomyces viscosus (Comb, nov.); a catalase-positive, facultative member of the genus Actinomyces . International Journal of Systematic Bacteriology 19:291–293
     [Google Scholar]
  21. Gilmour M. N., Beck P. H. 1961; The classification of organisms termed Leptotrichia (Leptothrix) buccalis. III. Growth and biochemical characteristics of Bacterionema matruchotii . Bacteriological Reviews 25:152–161
     [Google Scholar]
  22. Gilmour M. N., Howell A. Jun., Bibby B. G. 1961a; The classification of organisms termed Leptotrichia (Leptothrix) buccalis. I. Review of the literature and proposed separation into Leptotrichia buccalis Trevisan, 1879) and Bacterionema gen.nov., B. matruchotii (Mendel, 1917). comb.nov. Bacteriological Review 25:131–141
     [Google Scholar]
  23. Gilmour M. N., Howell A. Jun., Bibby B. G. 1961b; Proposal for designation of neotype strains of Septotrichia buccalis and Bacterionema matruchotii . International Bulletin of Bacteriological Nomenclature and Taxonomy 11:161–164
     [Google Scholar]
  24. Gordon R. E. 1967; The taxonomy of soil bacteria. In The Ecology of Soil Bacteria Edited by Gray T. R. G., Parkinson D. Liverpool: Liverpool University Press;
     [Google Scholar]
  25. Gordon R. E., Mihm J. M. 1957; A comparative study of some strains received as nocardiae. Journal of Bacteriology 73:15–27
     [Google Scholar]
  26. Gordon R. E., Mihm J. M. 1959; A comparison of four species of mycobacteria. Journal of General Microbiology 21:736–748
     [Google Scholar]
  27. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria. Journal of General Microbiology 69:33–80
     [Google Scholar]
  28. Gower J. C., Ross G. J. S. 1969; Minimum spanning trees and single-linkage cluster analysis. Applied Statistics 18:54–56
     [Google Scholar]
  29. Grüter W. 1932; Eine Pilzgeschwulst (Aktinomykose) in oberen Tränenröhrchen (Actinomyces discofoliatus). Zeitschrift für Augenheilkunde 79:477–510
     [Google Scholar]
  30. Hammond B. F. 1970a; Isolation and serological characterization of a cell wall antigen of Rothia dentocariosa . Journal of Bacteriology 103:634–640
     [Google Scholar]
  31. Hammond B. F. 1970b; Deoxyribonucleic acid base composition of Rothia dentocariosa as determined by thermal denaturation. Journal of Bacteriology 103:1024–1026
     [Google Scholar]
  32. Harrington B. J. 1964 The Taxonomy of Oral Corynebacteria Thesis, University of Birmingham;
     [Google Scholar]
  33. Harrington B. J. 1966; A numerical taxonomical study of some corynebacteria and related organisms. Journal of General Microbiology 45:31–40
     [Google Scholar]
  34. Hill L. R., Turri M., Gilardi E., Silverstri L. 1961; Quantitative methods in the systematics of Actinomycetales. II. General Microbiology 9:56–72
     [Google Scholar]
  35. Holding A. J., Collee J. G. 1971; Routine biochemical tests. In Methods in Microbiology pp 1–32 Edited by Norris J. R., Ribbons D. W. London and New York: Academic Press;
     [Google Scholar]
  36. Holmberg K., Forsum U. 1973; Identification of Actinomycetes, Arachnia, Bacterionema, Rothia and Proprionibacterium species by defined immunofluorescence. Applied Microbiology (In the press.)
    [Google Scholar]
  37. Holmberg K., Hallander H. O. 1972; Interference between Gram-positive microorganisms in dental plaque. Journal of Dental Research 51:588–595
     [Google Scholar]
  38. Howell A., Jordan H. V., Georg L. K., Pine L. 1965; Odontomyces viscosus, gen. nov., spec, nov., a filamentous microorganism isolated from periodental plaque in hamsters. Sabouraudia 4:65–68
     [Google Scholar]
  39. Howell A., Pine L. 1961; The classification of organisms termed Leptotrichia (Leptothrix) buccalis. IV. Physiological and biochemical characteristics of Bacterionema matruchotii . Bacteriological Reviews 25:162–171
     [Google Scholar]
  40. Howell A., Stephan R. M., Paul F. 1962; Prevalence of Actinomyces israelii, A. naeslundii, Bacterionema matruchotti and Candida albicans in selected areas of the oral cavity and saliva. Journal of Dental Research 41:1050–1059
     [Google Scholar]
  41. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. Journal of Bacteriology 66:24–26
     [Google Scholar]
  42. Jardine N., Sibson R. 1971 Mathematical Taxonomy London: John Wiley and Sons;
     [Google Scholar]
  43. Jordan H. V., Howell A. 1965; Nutritional control of cellular morphology in an aerobic actinomycete from the hamster. Journal of General Microbiology 38:125–130
     [Google Scholar]
  44. Jordan H. V., Keyes P. H., Bellack S. 1972; Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin. Journal of Periodontal Research 7:21–28
     [Google Scholar]
  45. Krämer J., Schlegel H. G. 1967; Die Bedeutung der Ureaserepression für die taxonomische Klassi-fizierung von Bakterien. Zentralblatt für Bakteriologie (Abteilung II) 121:414–423
     [Google Scholar]
  46. Lechevalier H. A., Lechevalier M. P. 1967; Biology of actinomycetes. Annual Review of Microbiology 21:71–100
     [Google Scholar]
  47. Liston J., Wiebe W., Colwell R. R. 1963; Quantitative approach to the study of bacterial species. Journal of Bacteriology 85:1061–1070
     [Google Scholar]
  48. Lockhart W. R., Liston J. Eds 1970 Methods for Numerical Taxonomy Bethesda, Maryland: American Society for Microbiology;
     [Google Scholar]
  49. Melville T. H. 1965; A study of the overall similarity of certain actinomycetes mainly of oral origin. Journal of General Microbiology 40:309–315
     [Google Scholar]
  50. Moore K., Davis G. H. G. 1963; Taxonomy and incidence of oral corynebacteria. British Dental Journal 114:254–258
     [Google Scholar]
  51. Morris E. O. 1954; The bacteria of the oral cavity. V. Corynebacteria and Gram-positive filamentous organisms. British Dental Journal 97:29–34
     [Google Scholar]
  52. Onisi M. 1949; Study on the Actinomyces isolated from the deeper layers of carious dentine. Shikagaku Zasshi 6:273–282
     [Google Scholar]
  53. Pine L. 1970; Classification and phylogenetic relationship of microaerophilic actinomycetes. International Journal of Systematic Bacteriology 20:445–474
     [Google Scholar]
  54. Pine L., Georg L. K. 1965; The classification and phylogenetic relationship of the Actinomycetales . International Bulletin of Bacteriological Nomenclature and Taxonomy 15:153–164
     [Google Scholar]
  55. Pine L., Georg L. K. 1969; Reclassification of Actinomyces propionicus . International Journal of Systematic Bacteriology 19:267–272
     [Google Scholar]
  56. Rasmussen E. G., Gibbons R. J., Socransky S. S. 1966; A taxonomic study of fifty Gram-positive anaerobic diphtheriods isolated from the oral cavity of man. Archives of Oral Biology 11:573–579
     [Google Scholar]
  57. Roth G. D. 1957; Proteolytic organisms of the carious lesion. Oral Surgery, Oral Medicine and Oral Pathology 10:1105–1117
     [Google Scholar]
  58. Roth G. D., Flanagan V. 1969; The pathogenicity of Rothia dentocariosa inoculated into mice. Journal of Dental Research 48:957–958
     [Google Scholar]
  59. Roth G. D., Thurn A. N. 1962; Continued study of oral Nocardia . Journal of Dental Research 41:1279–1292
     [Google Scholar]
  60. Slack J. M. 1968; Subgroup on Taxonomy of Microaerophilic Actinomycetes. Report on organization, aims and procedures. International Journal of Systematic Bacteriology 18:253–262
     [Google Scholar]
  61. Slack J. M., Gerencser M. A. 1970a; The genus Actinomyces . In The Actinomycetales pp 19–27 Edited by Prauser H. Jena: G. Fischer Verlag;
     [Google Scholar]
  62. Slack J. M., Gerencser M. A. 1970b; Two new serological groups of Actinomyces . Journal of Bacteriology 103:266–267
     [Google Scholar]
  63. Slack J. M., Landfried S., Gerencser M. A. 1969; Morphological, biochemical and serological studies on 64 strains of Actinomyces israelii . Journal of Bacteriology 97:873–884
     [Google Scholar]
  64. Smith R. F. 1969; Characterization of human cutaneous lipophilic diphtheroids. Journal of General Microbiology 55:433–443
     [Google Scholar]
  65. Sneath P. H. A. 1957a; Some thoughts on bacterial classification. Journal of General Microbiology 17:184–200
     [Google Scholar]
  66. Sneath P. H. A. 1957b; The application of computers to taxonomy. Journal of General Microbiology 17:201–226
     [Google Scholar]
  67. Snyder M. L., Bullock W. W., Parker R. B. 1967; Morphology of Gram-positive filamentous bacteria identified in dental plaque by fluorescent antibody technique. Archives of Oral Biology 12:1269–1273
     [Google Scholar]
  68. Snyder M. L., Slawson M. S., Bullock W., Parker R. B. 1967; Studies on oral filamentous bacteria. II. Serological relationships within the genera Actinomyces, Nocardia, Bacterionema and Leptotrichia . Journal of Infectious Diseases 117:341–345
     [Google Scholar]
  69. Socransky S. S., Hubersak C., Propas D. 1970; Induction of periodontal destruction in gnotobiotic rats by human oral strain of Actinomyces naeslundii . Archives of Oral Biology 15:993–995
     [Google Scholar]
  70. Sokal R. R., Michener C. D. 1958; A statistical method for evaluating systematic relationships. Kansas University Science Bulletin 38:1409–1438
     [Google Scholar]
  71. Sokal R. S., Sneath P. H. A. 1963 Principles of Numerical Taxonomy San Francisco: W. H. Freeman;
     [Google Scholar]
  72. Streckfuss J. L., Smith W. N. 1970; Isolation of bacillary and streptococcal variants from Bacterionema matruchotii . Journal of Bacteriology 104:1399–1400
     [Google Scholar]
  73. Thompson L., Lovestedt S. A. 1951; An actinomyces-like organism obtained from the human mouth. Mayo Clinic Proceedings 26:169–175
     [Google Scholar]
  74. Tsukamura M. 1967; Identification of mycobacteria. Tubercle 48:311–338
     [Google Scholar]
  75. Tsukamura M. 1969; Numerical taxonomy of the genus Nocardia . Journal of General Microbiology 56:265–287
     [Google Scholar]
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Numerical Taxonomy and Laboratory Identification of Bacterionema matruchotii, Rothia dentocariosa, Actinomyces naeslundii, Actinomyces viscosus, and Some Related Bacteria
Microbiology 76, 43 (1973); https://doi.org/10.1099/00221287-76-1-43
/content/journal/micro/10.1099/00221287-76-1-43
/content/journal/micro/10.1099/00221287-76-1-43
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