1887

Abstract

SUMMARY: When mycelia of are grown with lactose or galactose as the carbon source, -galactosidase activity is induced at least 30-fold. The effect of growth conditions on the formation of the activity was investigated.

The enzyme has been partially characterized: two proteins with molecular weights near 120000 and 450000 have -galactosidase activity; present evidence suggests that these are different aggregates of the same polypeptide.

A mutant, 150, is described which grows poorly on lactose and lacks -galactosidase activity. These two characters are determined by two unlinked genes and independently expressed. This demonstrates the existence of an alternative (and unknown) mode of lactose utilization. Although -galactosidase activity is not required for mycelial growth on lactose, it is essential for germination of conidia with lactose as the sole carbon source.

Mutants unable to form -galactosidase activity fall into three genetic groups designated and All these mutants grow normally with lactose as sole carbon source. One mutant at the locus forms a heat-labile -galactosidase and is presumably a structural gene lesion.

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/content/journal/micro/10.1099/00221287-77-2-471
1973-08-01
2024-04-30
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