1887

Abstract

SUMMARY: The lipopolysaccharides from fifteen strains of used for serotyping have been isolated and analysed. They all contained heptose, glucose, rhamnose, glucosamine, galactosamine, 2-keto-3-deoxyoctonic acid and alanine. Many of them contained additional sugars and amino compounds, some of which have been shown to be amino sugars. Their composition and structure suggested the existence of a common region of low molecular weight in all the lipopolysaccharides. They could be classified by their chemistry and that of their degradation products into twelve chemogroups, nine of which contained one serotype and three of which contained two serotypes. All the lipopolysaccharides contained the same fatty acids, 3-OH 10:0, 2-OH 12:0, 3-OH 12:0 and 12:0. The isolated lipopolysaccharides reacted with the homologous Habs type antisera. The serological specificity was limited to the high molecular weight regions thought to correspond to side chains.

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1973-10-01
2024-05-01
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References

  1. Adam M. M., Kontrohr T., Horvath E. 1971; Serological studies on Pseudomonas aeruginosa O group lipopolysaccharides.. Acta microbiologica Academiae scientiarum hungaricae 18:307–317
    [Google Scholar]
  2. Ashwell G., Hickman J. 1971; The chemistry of the unique carbohydrates of bacterial lipopolysaccharides. In Microbial Toxins vol Iv pp 235–266 Edited by Weinbaum T., Kadis S., Ajl S. J. New York: Academic Press;
    [Google Scholar]
  3. Bartlett G. R. 1959; Phosphorus assay in column chromatography.. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  4. Chester I. R., Gray G. W., Wilkinson S. G. 1972; Further studies of the chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa.. Biochemical Journal 126:395–407
    [Google Scholar]
  5. Drewry D. T., Gray G. W., Wilkinson S. G. 1971; Release of ethanolamine pyrophosphate during mild acid hydrolysis of the lipopolysaccharide of Pseudomonas aeruginosa.. European Journal of Bio-chemistry 21:400–403
    [Google Scholar]
  6. Drewry D. T., Gray G. W., Wilkinson S. G. 1972a; Low molecular weight solutes released during mild acid hydrolysis of the lipopolysaccharides of Pseudomonas aeruginosa.. Biochemical Journal 130:289–295
    [Google Scholar]
  7. Drewry D. T., Gray G. W., Wilkinson S. G. 1972b; Structural studies of a lipopolysaccharide from Pseudomonas aeruginosa.. Journal of General Microbiology 73:viii
    [Google Scholar]
  8. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances.. Analytical Chemistry 28:350–356
    [Google Scholar]
  9. Ellwood D. C., Tempest D. W. 1972; Effects of environment on bacterial wall content and composition. In Advances in Microbial Physiology vol 7 pp 83–117 Edited by Rose A. H., Tempest D. W. London: Academic Press.;
    [Google Scholar]
  10. Fensom A. H., Gray G. W. 1969; The chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa.. Biochemical Journal 114:185–196
    [Google Scholar]
  11. Fensom A. H., Meadow P. M. 1970; Evidence for two regions in the polysaccharide moiety of the lipopolysaccharide o.. Pseudomonas aeruginosa 8602 FEBS Letters 9:81–84
    [Google Scholar]
  12. Habs I. 1957; Untersuchungen über die O-Antigene von Pseudomonas aeruginosa.. Zeitschrift für Hygiene and Infektionskrankheiten 144:218–228
    [Google Scholar]
  13. Hancock I. C., Humphreys G. O., Meadow P. M. 1969; The hydroxyacids o.. Pseudomonas aeruginosa 8602 Biochemical Journal 113:30 P
    [Google Scholar]
  14. Hancock I. C., Humphreys G. O., Meadow P. M. 1970; Characterisation of the hydroxyacids o.Pseudomonas aeruginosa; 8602 Biochimica et biophysica acta 202:389–391
    [Google Scholar]
  15. Hanessian S., Regan W., Watson D., Haskell T. H. 1971; Isolation and characterisation of antigenic components of a new heptavalent Pseudomonas vaccine.. Nature New Biology, London 229:209–210
    [Google Scholar]
  16. Humphreys G. O., Hancock I. C., Meadow P. M. 1972; Synthesis of the hydroxyacids in lipid A of Pseudomonas aeruginosa.. Journal of General Microbiology 71:221–230
    [Google Scholar]
  17. Ikeda K., Egami F. 1973; Lipopolysaccharide of Pseudomonas aeruginosa with special reference to pyocin R receptor activity.. Journal of General and Applied Microbiology 19:115–128
    [Google Scholar]
  18. Kauffmann F. 1966 The Bacteriology of Enterobacteriaceae Copenhagen: Munksgaard.;
    [Google Scholar]
  19. Key B. A., Gray G. W., Wilkinson S. G. 1970; The purification and chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa.. Biochemical Journal 120:559–566
    [Google Scholar]
  20. Lamkin W. M., Ward D. N., Walborg E. F. 1966; Quantitative determination of neutral monosaccharide residues in glycoproteins and glycopeptides by thin layer densitometry.. Analytical Biochemistry 17:485–494
    [Google Scholar]
  21. Lancefield R. C. 1933; A serological differentiation of human and other groups of haemolytic streptococci.. Journal of Experimental Medicine 57:571–595
    [Google Scholar]
  22. Lüderitz O., Jann K., Wheat R. 1968; Somatic and capsular antigens of Gram-negative bacteria. In Comprehensive Biochemistry vol 26a pp 105–228 Edited by Florkin M., Stotz E. H. Amsterdam: Elsevier.;
    [Google Scholar]
  23. Lüderitz O., Staub A. M., Westphal O. 1966; Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae.. Bacteriological Reviews 30:192–255
    [Google Scholar]
  24. Lüderitz O., Westphal O., Staub A. M., Nikaido H. 1971; Isolation and chemical and immunological characterisation of bacterial lipopolysaccharides. In Microbial Toxins vol Iv pp 331–350 Edited by Weinbaum T., Kadis S., Ajl S. J. New York: Academic Press;
    [Google Scholar]
  25. Osborn M. J. 1963; Studies on the Gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Pseudomonas aeruginosa.. Proceedings of the National Academy of Sciences of the United States of America 50:499–506
    [Google Scholar]
  26. Ouchterlony O. 1953; Antigen-antibody reactions in gels.. Acta pathologica et microbiologica scandinavica 26:507–515
    [Google Scholar]
  27. Sandvik O. 1960; Serological comparison between strains of Pseudomonas aeruginosa from human and animal sources.. Acta pathologica et microbiologica scandinavica 48:56–60
    [Google Scholar]
  28. Suzuki N. 1969; Isolation of dl-fucosamine from Pseudomonas aeruginosa n 10.. Biochimica et biophysica acta 177:371–373
    [Google Scholar]
  29. Suzuki N., Suzuki A., Fukasawa K. 1970; Distribution of 2-amino sugars in Pseudomonas aeruginosa-, isolation of d-quinovosamine from Pseudomonas aeruginosa P14.. Journal of the Japanese Biochemical Society 42:130–134
    [Google Scholar]
  30. Swift H. F., Wilson A. T., Lancefield R. C. 1943; Typing group A haemolytic streptococci by M precipitin reactions in capillary pipettes.. Journal of Experimental Medicine 78:127–133
    [Google Scholar]
  31. Veron M. 1961; Sui l’agglutination de Pseudomonas aeruginosa: subdivision des groupes antigeniques 0:2 et 0:5.. Annales de l’Institut Pasteur 101:456–460
    [Google Scholar]
  32. Westphal O., Jann O. 1965; Bacterial lipopolysaccharides. In Methods in Carbohydrate Chemistry vol 5 pp 83–91 Edited by Whistler R. L. New York: Academic Press.;
    [Google Scholar]
  33. Wilkinson S. G. 1968; Studies on the cell walls of Pseudomonas species resistant to ethylenediaminetetra- acetic acid.. Journal of General Microbiology 54:195–213
    [Google Scholar]
  34. Wilkinson S. G. 1970; Cell walls of Pseudomonas species sensitive to ethylenediaminetetra-acetic acid.. Journal of Bacteriology 104:1035–1044
    [Google Scholar]
  35. Wilkinson S. G. 1972; Amino sugars in the wall of Pseudomonas aeruginosa.. Journal of General Microbiology 70:365–369
    [Google Scholar]
  36. Wilkinson S. G., Carby K. A. 1971; Amino sugars in the cell walls of Pseudomonas species.. Journal of General Microbiology 66:221–227
    [Google Scholar]
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