1887

Microbiology Society logo

Volume 80, Issue 1

Characterization of a Conditional Mutant with Altered Envelope Showing pH-dependent Morphology and Temperature-dependent Division Free

Abstract

SUMMARY: The properties of a conditional mutant of (Mir M7) are described. The mutant has a pH-dependent disturbance of morphology and a temperature-dependent disturbance of division. At pH 7 the mutant grows as cocci and at 41 °C (restrictive temperature) division is inhibited. At acid pH and restrictive temperature, filaments are formed. At pH 7 and permissive temperature, polymorphous cocci are obtained. At pH 7 and 41 °C, giant polymorphous cells are formed. The inhibition of division is phenotypically reverted by 0·05 -Mg, 0·1 -Na or 0·4 -sucrose. Seventeen revertants were isolated having exactly the same characteristics as the wild-type , so it seems that only one mutation is responsible for the different disturbances of the mutant. The possibility of membrane damage on which the morphology and division disturbances may depend is discussed.

  • Received:
  • Published Online:
© Society for General Microbiology, 1974
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-80-1-51
1974-01-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/80/1/mic-80-1-51.html?itemId=/content/journal/micro/10.1099/00221287-80-1-51&mimeType=html&fmt=ahah

References

  1. Boylan R. J., Mendelson N. H. 1969; Initial characterization of a temperature-sensitive rod− mutant of Bacillus subtilis. Journal of Bacteriology 100:1316–1321
     [Google Scholar]
  2. Cohen G. N., Monod J. 1957; Bacterial permeases. Bacteriological Reviews 21:169–194
     [Google Scholar]
  3. Cohen S. S., Lichtenstein J. 1960; Polyamines and ribosome structure. Journal of Biological Chemistry 235:776–782
     [Google Scholar]
  4. Cole R. M., Popkin T. J., Boylan R. J., Mendelson N. H. 1970; Ultrastructure of a temperature- sensitive rod− mutant of Bacillus subtilis. Journal of Bacteriology 103:793–810
     [Google Scholar]
  5. Donachie W. D., Begg K. J. 1970; Growth of the bacterial cell. Nature; London: 2271220–1224
     [Google Scholar]
  6. Grula E. A., King R. D. 1970; Inhibition of cell division in Micrococcus lysodeikticus dis. II. Canadian Journal of Microbiology 16:317–324
     [Google Scholar]
  7. Hirota Y., Mordoh J., Jacob F. 1970; On the process of cellular division in Escherichia coli. HI. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. Journal of Molecular Biology 53:369–387
     [Google Scholar]
  8. Hirota Y., Ryter A., Jacob F. 1968; Thermosensitive mutants of Escherichia coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harbor Symposia on Quantitative Biology 33:677–693
     [Google Scholar]
  9. Hirota Y., Wakil S., Shapiro B., Ryter A., Hurwitz J., Jacob F. 1969; Sur un mutant thermosensible d’Escherichia coli présentant des anomalies de la membrane. Comptesrendus de l’Academie des sciences D269:1346–1348
     [Google Scholar]
  10. Holland E. M., Holland I. H. 1970; Induction of DNA breakdown and inhibition of cell division by colicin E2. Nature of some early steps in the process and properties of the E2-specific nuclease system. Journal of General Microbiology 64:223–239
     [Google Scholar]
  11. Inouye M., Pardee A. B. 1970; Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli. Journal of Biological Chemistry 245:5813–5819
     [Google Scholar]
  12. Jacob F., Brenner S., Cuzin F. 1963; On the regulation of DNA replication in bacteria. Cold Spring Harbor Symposia on Quantitative Biology 28:329–337
     [Google Scholar]
  13. MacQuillen K. 1960; Bacterial protoplasts. In The Bacteria 1 pp. 249–359 Gunsalus I. C., Stanier R. Y. Edited by New York: Academic Press;
     [Google Scholar]
  14. Mager J. 1959a; Spermine as a protective agent against osmotic lysis. Nature; London: 1831827–1828
     [Google Scholar]
  15. Mager J. 1959b; The stabilizing effect of spermine and related polyamines on bacterial protoplasts. Biochimica et biophysica acta 36:529–531
     [Google Scholar]
  16. Meloni G. A., Monti-Bragadin C. 1962; Osservazioni sull’isolamento dall’uomo di sferoplasti ‘spontanei’ derivati da un ceppo di Klebsiella. Annali Sclavo 4:143–152
     [Google Scholar]
  17. Nagel de Zwaig R., Luria S. E. 1967; Genetics and physiology of colicin-tolerant mutants of Escherichia coli. Journal of Bacteriology 94:1112–1123
     [Google Scholar]
  18. Puck C. W. 1960; The stabilizing effect of spermine and related amines on mitochondria and protoplasts. Biochemical and Biophysical Research Communications 2:117–120
     [Google Scholar]
  19. Rogers H. J. 1970; Bacterial growth and the cell envelope. Bacteriological Reviews 34:194–214
     [Google Scholar]
  20. Rogers H. J., MacConnell R., Burdett J. D. 1970; Isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and division cell. Journal of General Microbiology 61:155–171
     [Google Scholar]
  21. Rolfe B., Onodera K. 1971; Demonstration of missing membrane proteins in colicin-tolerant mutant of E. coli K12. Biochemical and Biophysical Research Communications 44:767–773
     [Google Scholar]
  22. Ryter A. 1967; Relationship between synthesis of the cytoplasmic membrane and nuclear segregation in Bacillus subtilis. Acta microbiologica Academiae scientiarum hungaricae 12:284–290
     [Google Scholar]
  23. Ryter A. 1971; Étude de la croissance de la membrane chez Bacillus subtilis au moyen de la distribution des flagelles. Annales de l’Institut Pasteur de Lille 121:271–288
     [Google Scholar]
  24. Samson A. C. R., Holland I. B. 1970; Envelope protein changes in mutants OF Escherichia coli refractory to colicin E2. FEBS letters 11:33–36
     [Google Scholar]
  25. Satta G., Fontana R. 1974; Cell division, macromolecular synthesis and morphology dependent on the state of the envelope in a mutant of Klebsiella pneumoniae. Journal of General Microbiology 80:65–75
     [Google Scholar]
  26. Satta G., Schito G. C., Meloni G. A. 1969; Transizione bastoncinosfera in un ceppo di Klebsiella pneumoniae. Ultrastruttura delle forme coccoidi tipiche ed abnormi. Atti del XV° Congresso Nazionale di Microbiologia Torina-Saint Vincent 2: pp. 247–253
     [Google Scholar]
  27. Schachtele C. F., Anderson D. L., Rogers P. 1968; Mechanism of canavanine death in Escherichia coli. II. Membrane-bound canavanyl-protein and nuclear disruption. Journal of Molecular Biology 33:861–872
     [Google Scholar]
  28. Schwarz U., Asmus A., Frank H. 1969; Autolytic enzymes and cell division of Escherichia coli. Journal of Molecular Biology 41:419–429
     [Google Scholar]
  29. Shapiro B. M., Siccardi A. G., Hirota Y., Jacob F. 1970; On the process of cellular division in Escherichia coli. II. Membrane protein alterations associated with mutations affecting the initiation of DNA synthesis. Journal of Molecular Biology 52:75–89
     [Google Scholar]
  30. Siccardi A. G., Shapiro B. M., Hirota Y., Jacob F. 1971; On the process of cellular division in Escherichia coli. IV. Altered composition and turnover of the membranes of thermosensitive mutants defective in chromosomal replication. Journal of Molecular Biology 56:475–490
     [Google Scholar]
  31. Walker J. R., Pardee A. B. 1968; Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli. Journal of Bacteriology 95:123–131
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-80-1-51
Loading
Characterization of a Conditional Mutant with Altered Envelope Showing pH-dependent Morphology and Temperature-dependent Division
Microbiology 80, 51 (1974); https://doi.org/10.1099/00221287-80-1-51
/content/journal/micro/10.1099/00221287-80-1-51
/content/journal/micro/10.1099/00221287-80-1-51
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error