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Volume 82, Issue 1

Effect of Potassium Cyanide, Glucose and Anaerobiosis on Morphogenesis of Free

Abstract

Summary: The morphological effect of potassium cyanide on the aerobic germination of spores of (1894) in the presence of glucose was investigated. At concentrations of up to 2 m-KCN, normal filaments were formed; at concentrations of 3 to 6 m intermediate forms were observed ranging from enlarged and septated hyphae to rounded multipolar cells; at 8 m spores produced only budding spherical cells. The effect of glucose on the anaerobic germination of spores was also studied. At 0·01 % glucose hyphal development occurred, whereas at increasing concentrations dimorphic colonies were formed. At 2 % glucose purely yeast colonies appeared of the type described by Bartnicki-García (1968) for 8o. In the absence of glucose, or when glucose was replaced by xylose, maltose or succinate, spores did not germinate.

Anaerobiosis did not prevent the elongation of preformed hyphae obtained under aerobic conditions, provided that the cellular integrity was maintained. The anaerobic mycelia had a fermentative metabolism, as indicated by the low level of cytochrome oxidase, the amount and the type of pyruvate kinase, and the production of ethyl alcohol. Conversion of hyphal to yeast-like morphology was observed when tips of young hyphae were cultured in conditions favouring fermentation. The budding cells obtained were indistinguishable from yeast-like cells generated by anaerobic germination of spores.

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© Society for General Microbiology 1974
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1974-05-01
2024-05-06
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Effect of Potassium Cyanide, Glucose and Anaerobiosis on Morphogenesis of Mucor rouxii
Microbiology 82, 15 (1974); https://doi.org/10.1099/00221287-82-1-15
/content/journal/micro/10.1099/00221287-82-1-15
/content/journal/micro/10.1099/00221287-82-1-15
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