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Volume 83, Issue 2

Genetic Transformation in Yeasts Free

Abstract

SUMMARY: The transformability of 25 strains of yeasts belonging to 23 species in three genera - and - was investigated with respect to ten characters. Eighteen hour cultures at about the end of the log phase of growth, addition of 10 g DNA/ml, and an incubation time of 30 min at 30 to 37 °C usually yielded best results. Of the 109 pairs of strains investigated 11 yielded positive results, which included intraspecific, interspecific and intergeneric transformation. Three adenine-requiring mutants of were transformed to prototrophy with the DNA of S. var. . DNA of the latter strain also transformed three other species of Saccharomyces. In Candida, reciprocal transformation was observed between C. and transformed to rhamnose utilization. In intergeneric experiments, was transformed with var. DNA and C. with the DNA from . Attempts at transformation between Saccharomyces and Candida were unsuccessful. The GC content of DNA from the strains which yielded positive results varied from 36·0 to 37·3 mol %. The implications of these findings for the inter-relationships and taxonomy of yeasts are discussed.

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© Society for General Microbiology 1974
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1974-08-01
2024-04-30
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Genetic Transformation in Yeasts
Microbiology 83, 237 (1974); https://doi.org/10.1099/00221287-83-2-237
/content/journal/micro/10.1099/00221287-83-2-237
/content/journal/micro/10.1099/00221287-83-2-237
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