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Arginine Analogues: Effect on Growth and on the First Two Enzymes of the Arginine Pathway in Pseudomonas aeruginosa
Abstract
Summary: The effect of 14 arginine analogues on the growth of Pseudomonas aeruginosa in a liquid mineral medium with either l-glutamate or l-ornithine as the sole carbon and nitrogen source was investigated. Two analogues, l-indospicine and l-arginine hydroxamate, inhibited growth markedly. The former was most effective in the or-nithine medium and the latter only in the glutamate medium.
The inhibition by arginine analogues of the first two enzymes of the arginine pathway, N-acetylglutamate synthetase and N-acetylglutamate 5-phosphotransferase, was studied. It was found that inhibition of these two feedback-sensitive enzymes did not correlate with inhibition of growth. O-(l-norvalyl-5)-isourea, which was a rather weak inhibitor of growth, was at least three times more active as an inhibitor of the enzymes than l-indospicine and l-arginine hydroxamate. These two analogues were about ten times less effective than l-arginine, the strongest inhibitor of both enzymes.
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© Society for General Microbiology, 1974