1887

Microbiology Society logo

Volume 84, Issue 2

Lysogeny in Lactobacilli Free

Abstract

Summary: Strains of lactobacilli were examined for lysogeny after treatment with mitomycin C. Forty strains belonging to 7 species out of 148 strains of 15 species were lysed by mitomycin C. Lysis was strongly dependent upon the concentration of mitomycin C, temperature and the age of the cultures.

Phage-like particles were observed in 31 out of 40 lysates by electron microscopy. Particles from ten lysates – four from B, five from C and one from – produced plaques when plated on suitable indicator strains and were thus considered to be active phages. The remaining 21 lysates that contained phage-like particles failed to produce plaques but four of them inhibited the growth of other lactobacilli. It was concluded that the particles in these lysates were defective phages.

Activities, shapes, dimensions and serological properties of the newly induced phage-like particles were also studied.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1974
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-84-2-277
1974-10-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/84/2/mic-84-2-277.html?itemId=/content/journal/micro/10.1099/00221287-84-2-277&mimeType=html&fmt=ahah

References

  1. Coetzee J. N., de Klerk H. C. 1962; Lysogeny in the genus Lactobacillus . Nature, London 194:505
     [Google Scholar]
  2. Efthymiou C., Hansen P. A. 1962; An antigenic analysis of Lactobacillus acidophilus . Journal of Infectious Diseases 110:258–267
     [Google Scholar]
  3. Ishiwa H., Sakurai T. 1972; Induction procedures of lytic enzymes and temperature phages of lysogenic strains of group C. Lactobacillus. Nippon Nogei Kagaku Kaishi 46:245–251
     [Google Scholar]
  4. de Klerk H. C., Coetzee J. N., Fourie J. T. 1965; The fine structure of lactobacillus bacteriophages. Journal of General Microbiology 38:35–38
     [Google Scholar]
  5. de Klerk H. C., Hugo N. 1970; Phage-like structures from Lactobacillus acidophilus . Journal of General Virology 8:231–234
     [Google Scholar]
  6. de Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for cultivation of lactobacilli. Journal of Applied Bacteriology 23:130–135
     [Google Scholar]
  7. Murata A., Soeda E., Saruno R. 1969; Factors influencing plaque formation by bacteriophage of Lactobacillus acidophilus . Nippon Nogei Kagaku Kaishi 43:311–316
     [Google Scholar]
  8. Otsuji N., Sekiguchi M., Iijima T., Takagi Y. 1959; Induction of phage formation in the lysogenic Escherichia coli k-12 by mitomycin C. Nature, London 184:1079–1080
     [Google Scholar]
  9. Rogosa M., Sharpe M. E. 1959; An approach to the classification of the lactobacilli. Journal of Applied Bacteriology 22:329–340
     [Google Scholar]
  10. Sakurai T., Takahashi T., Arai H. 1970; The temperate phages of Lactobacillus salivarius and Lactobacillus casei . Japanese Journal of Microbiology 14:333–336
     [Google Scholar]
  11. Sakurai T., Tohyama K., Arai H. 1971; Properties of a temperate phage of Lactobacillus salivarius . Japanese Journal of Bacteriology 26:473–481
     [Google Scholar]
  12. Seaman E., Tarmy E., Marmur J. 1964; Inducible phages of Bacillus subtilis . Biochemistry 3:607–613
     [Google Scholar]
  13. Sharpe M. E. 1955; A serological classification of lactobacilli. Journal of General Microbiology 12:107–122
     [Google Scholar]
  14. Tohyama K., Sakurai T., Arai H., Oda A. 1972; Studies on temperate phages of Lactobacillus salivarius . Japanese Journal of Microbiology 16:385–395
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-84-2-277
Loading
Lysogeny in Lactobacilli
Microbiology 84, 277 (1974); https://doi.org/10.1099/00221287-84-2-277
/content/journal/micro/10.1099/00221287-84-2-277
/content/journal/micro/10.1099/00221287-84-2-277
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error