1887

Abstract

SUMMARY

The effect of various solubilizing agents on the release of the dextranase from whole Cytophaga bacteria or washed envelopes is described. Among the proteolytic enzymes tested, only α-chymotrypsin solubilized the dextranase very efficiently with retained enzymic activity even after prolonged exposure. Among the snake venoms tested, that of showed the highest selectivity and efficiency. Non-ionic detergents with hydrophilic-lipophilic balance values of about 13 to 15 also effectively and selectively solubilized the membrane-bound enzyme. Organic solvents, urea, guanidine hydrochloride, glycine, chaotropic ions, pH variations and mechanical disruption (including osmotic shock) were ineffective.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-88-2-209
1975-06-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/88/2/mic-88-2-209.html?itemId=/content/journal/micro/10.1099/00221287-88-2-209&mimeType=html&fmt=ahah

References

  1. Collins F. M. 1964; Cell wall composition of a marine Cytophaga. Australian Journal of Experimental Biology and Medical Sciences 42:263–265
    [Google Scholar]
  2. French C. S., Milner H. W. 1955; Disintegration of bacteria and small particles by high-pressure extrusion. In Methods in Enzymology 1 pp. 64–67 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  3. Fryklund L., Eaker D. 1973; The complete amino acid sequence of a non-neurotoxic hemolytic protein from the venom of Haemachatus haemachates. Biochemistry 12:661–667
    [Google Scholar]
  4. Hanstein W. G., Davis K. A., Hatefi Y. 1971; Water structure and the chaotropic properties of halo-acetates. Archives of Biochemistry and Biophysics 147:534–544
    [Google Scholar]
  5. Hatefi Y., Hanstein W. G. 1969; Solubilization of particulate proteins and nonelectrolytes by chaotropic agents. Proceedings of the National Academy of Sciences of the United States of America 62:1129–1136
    [Google Scholar]
  6. Von Hofsten B., Van Kley H., Eaker D. 1965; An extracellular proteolytic enzyme from a strain of Arthrobacter. II. Purification and chemical properties of the enzyme. Biochimica et biophysica acta 110:585–598
    [Google Scholar]
  7. Holowach J., Howe M. C., Laatsch R. H., Mcdougal D. B. Jun 1966; Effects of various anions and cations on the release of four dehydrogenases from brain mitochondria by a non-ionic detergent. Biochimica et biophysica acta 118:230–239
    [Google Scholar]
  8. Janson J. -C. 1975; Studies on dextran-degrading enzymes. Isolation and identification of a dextranase- producing strain of Cytophaga johnsonii and studies on the formation of surface-bound enzyme. Journal of General Microbiology 88:205–208
    [Google Scholar]
  9. Kagawa Y. 1972; Reconstitution of oxidative phosphorylation. Biochimica et biophysica acta 265:297–338
    [Google Scholar]
  10. Makonnen B., Porath J. 1968; Purification of an extracellular proteinase from Penicillium notatum. European Journal of Biochemistry 6:425–431
    [Google Scholar]
  11. Morton R. K. 1955; Methods of extraction of enzymes from animal tissues. In Methods in Enzymology 1 pp. 25–51 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  12. Neeman A., Kahane I., Razin S. 1971; Characterization of the mycoplasma membrane proteins. II. Solubilization and enzymic activities of Acholeplasma laidlawii membrane proteins. Biochimica et biophysica acta 249:169–176
    [Google Scholar]
  13. Nossal N. G., Heppel L. A. 1966; The release of enzymes by osmotic shock from Escherichia coli in exponential phase. Journal of Biological Chemistry 241:3055–3062
    [Google Scholar]
  14. Nozaki Y., Tanford C. 1963; The solubility of amino acids and related compounds in aqueous urea solutions. Journal of Biological Chemistry 238:4074–4081
    [Google Scholar]
  15. Penefsky H. S., Tzagoloff A. 1971; Extraction of water soluble enzymes and proteins from membranes. In Methods in Enzymology 22 pp. 204–219 Jakoby W. B. Edited by New York: Academic Press;
    [Google Scholar]
  16. Razin S. 1972; Reconstitution of biological membranes. Biochimica et biophysica acta 265:241–296
    [Google Scholar]
  17. Singer S. J., Nicolson G. L. 1972; The fluid mosaic model of the structure of cell membranes. Science; New York: 175720–731
    [Google Scholar]
  18. Stanier R. Y. 1947; Studies on non-fruiting myxobacteria. I. Cytophaga johnsonaen. sp., a chitin-decomposing myxobacterium. Journal of Bacteriology 53:297–315
    [Google Scholar]
  19. Tzagoloff A., Penefsky H. S. 1971; Extraction and purification of lipoprotein complexes from membranes. In Methods in Enzymology 22 pp. 219–230 Jakoby W. B. Edited by New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-88-2-209
Loading
/content/journal/micro/10.1099/00221287-88-2-209
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error