1887

Abstract

SUMMARY: Plasma membranes were isolated from the yeast and mycelial forms of as described previously ( Marriott, 1975 ) and examined for the presence of several enzymes. Measurement of specific activities showed enrichment of Mg-dependent and Na/K-stimulated Mg-dependent adenosine triphosphatase and mannan synthetase, in the plasma membrane fractions from both morphological forms of the organism. However, acid and alkaline phosphatase, NADH oxidase and 5′-nucleotidase showed no such specific location.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-89-2-345
1975-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/89/2/mic-89-2-345.html?itemId=/content/journal/micro/10.1099/00221287-89-2-345&mimeType=html&fmt=ahah

References

  1. Algranati I. D., Carminatti H., Cabib E. 1965; The enzymic synthesis of yeast mannan. Biochemical and Biophysical Research Communications 12:504–509
    [Google Scholar]
  2. Avers C., Federman M. 1968; The occurrence in yeast of cytoplasmic granules which resemble microbodies. Journal of Cell Biology 37:555–559
    [Google Scholar]
  3. Behrens N. H., Cabib E. 1968; The biosynthesis of mannan in Saccharomyces carlsbergensis. Journal of Biological Chemistry 243:502–509
    [Google Scholar]
  4. Cartledge T., Lloyd D. 1971; Subcellular fractionation by differential and zonal centrifugation of aerobically grown glucose-depressed Saccharomyces carlsbergensis. Biochemical Journal 126:381–393
    [Google Scholar]
  5. Chen P. S., Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  6. Emmelot P., Bos C. T., Benedetti E. L., Rumke P. H. 1964; Studies on plasma membranes. I. Chemical composition and enzyme content of plasma membranes isolated from rat liver. Biochimica et biophysica acta 90:126–145
    [Google Scholar]
  7. Gezelius K. 1966; Acid phosphatase in Dictyostelium discoideum. Physiologia plantarum 19:946–959
    [Google Scholar]
  8. Gezelius K., Wright B. E. 1965; Alkaline phosphatase in Dictyostelium discoideum. Journal of General Microbiology 38:309–327
    [Google Scholar]
  9. Jan Y. N. 1974; Properties and cellular localization of chitin synthetase in Phycornyces blakesleeanus. Journal of Biological Chemistry 249:1973–1979
    [Google Scholar]
  10. Kamat V. B., Wallach D. F. H. 1965; Separation and partial purification of plasma membrane fragments from Ehrlich ascites carcinoma microsomes. Science; New York: 1481343–1345
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. C., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. Marriott M. S. 1975; Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans. Journal of General Microbiology 86:115–132
    [Google Scholar]
  13. Matile P., Moor H., Mühlethaler K. 1967; Isolation and properties of plasmalemma in yeast. Archiv für Mikrobiologie 58:201–211
    [Google Scholar]
  14. Matile P., Wiemken A. 1967; The vacuole as the lysosome of the yeast cell. Archiv für Mikrobiologie 56:148–155
    [Google Scholar]
  15. Nurminen T., Oura E., Suomalainen H. 1970; The enzymic composition of the isolated cell wall and plasma membrane of baker’s yeast. Biochemical Journal 116:61–69
    [Google Scholar]
  16. Perlman P., Mahler H. 1970; Intracellular localization of enzymes in yeast. Archives of Biochemistry and Biophysics 136:245–259
    [Google Scholar]
  17. Schatz G. 1963; The isolation of possible mitochondrial precursor structures from aerobically grown baker’s yeast. Biochemical and Biophysical Research Communications 12:448–451
    [Google Scholar]
  18. Schatz G., Klima J. 1964; Triphosphopyridine nucleotide: cytochrome C reductase of Saccharomyces cerevisiae, a microsomal enzyme. Biochimica et biophysica acta 81:448–461
    [Google Scholar]
  19. Schibeci A., Rattray J. B. M., Kidby D. K. 1973; Isolation and identification of yeast plasma membrane. Biochimica et biophysica acta 311:15–25
    [Google Scholar]
  20. Strominger J. 1969; Penicillin-sensitive enzymatic reactions in bacterial cell wall synthesis. Harvey Lectures 64:179–213
    [Google Scholar]
  21. Wallach D. F. H., Kamat V. B. 1966; Preparation of plasma membrane fragments from ascites tumor cells. In Methods in Enzymology 8 pp. 164–172 Edited by Neufeld E., Ginsburg V. New York and London: Academic Press;
    [Google Scholar]
  22. Wheeler G., Michell R., Rose A. H. 1972; Phosphatidyl inositol kinase activity in Saccharomyces cerevisiae. Biochemical Journal 127:64P
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-89-2-345
Loading
/content/journal/micro/10.1099/00221287-89-2-345
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error