1887

Abstract

SUMMARY: Chitin synthase of was present in the ‘microsomal’ fraction (100000 precipitate), the ‘cell-wall’ fraction (2000 precipitate) and the ‘mitochondrial’ fraction (10000 g precipitate). The properties of the ‘microsomal’ enzyme were investigated. The pH optimum was between 5·8 and 6·2, and the temperature optimum was between 31 and 33 °C. The for UDP -acetyl--glucosamine was 1·8 m. The enzyme was stimulated by Mg and a slight stimulation was also effected by -acetyl--glucosamine. Soluble chitodextrins were inhibitory. A pH-dependent, heat-stable inhibitor of chitin synthase activity was present in the soluble cytoplasm from the mycelium. The effects of aeration and glucose concentration on enzyme production in growing cultures were also investigated; maximum specific activity of chitin synthase was associated with the cessation of exponential growth.

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1975-10-01
2024-05-02
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References

  1. Bartnicki-Garcia S., Lippman E. 1969; Fungal morphogenesis: cell wall construction in Mucor rouxii. Science; Washington: 165302–304
    [Google Scholar]
  2. Cabib E., Keller F. A. 1971; Chitin and yeast budding. Allosteric inhibition of chitin synthetase by a heat stable protein from yeast. Journal of Biological Chemistry 246:167–173
    [Google Scholar]
  3. Cabib E., Ulane R. 1973a; Chitin synthetase activating factor from yeast, a protease. Biochemical and Biophysical Research Communications 50:186–191
    [Google Scholar]
  4. Cabib E., Ulane R. 1973b; Yeast chitin synthetase. Separation of zymogen from its activating factor and recovery of the latter in the vacuole fraction. Journal of Biological Chemistry 248:1451–1458
    [Google Scholar]
  5. Camargo E. O., Dietrich C. P., Sonneborn D., Strominger J. L. 1967; Biosynthesis of chitin in spores and growing cells of Blastocladiella emersonii. Journal of Biological Chemistry 242:3121–3128
    [Google Scholar]
  6. Glaser L., Brown D. H. 1957; The synthesis of chitin in cell free extracts of Neurospora crassa. Journal of Biological Chemistry 228:729–742
    [Google Scholar]
  7. Gooday G. W. 1971; An autoradiographic study of hyphal growth of some fungi. Journal of General Microbiology 67:125–133
    [Google Scholar]
  8. Gooday G. W. 1972; The role of chitin synthetase in the elongation of fruiting bodies of Coprinus cinerus. Journal of General Microbiology 73:xxi
    [Google Scholar]
  9. Gooday G. W. 1973; Activity of chitin synthetase during the development of fruiting bodies of the toadstool Coprims cinerus. Biochemical Society Transactions 1:1105–1107
    [Google Scholar]
  10. Grove S. N., Bracker C. E. 1970; Protoplasmic organization of hyphal tips among fungi; vesicles and Spitzenkörper. Journal of Bacteriology 104:989–1009
    [Google Scholar]
  11. Jan Y. N. 1974; Properties and cellular localization of chitin synthetase in Phycomyces blakesleeanus. Journal of Biological Chemistry 249:1973–1979
    [Google Scholar]
  12. Jaworski E. G., Wang L. C., Carpenter W. D. 1965; Biosynthesis of chitin in cell-free extracts of Venturia inequalis. Phytopathology 55:1309–1312
    [Google Scholar]
  13. Keller F. A., Cabib E. 1971; Chitin and yeast budding: properties of chitin synthetase from Saccharomyces carlsbergensis. Journal of Biological Chemistry 246:160–166
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Mcmurrough I., Bartnicki-Garcia S. 1971; Properties of a particulate chitin synthetase from Mucor rouxii. Journal of Biological Chemistry 246:4008–4016
    [Google Scholar]
  16. Mcmurrough I., Bartnicki-Garcia S. 1973; Inhibition and activation of chitin synthesis by Mucor rouxii cell extracts. Archives of Biochemistry and Biophysics 158:812–816
    [Google Scholar]
  17. Mcmurrough I., Flores-Carreon A., Bartnicki-Garcia S. 1971; Pathway of chitin synthesis and cellular localization of chitin synthetase in Mucor rouxii. Journal of Biological Chemistry 246:3999–4007
    [Google Scholar]
  18. Moore P. M., Peberdy J. F. 1975; Biosynthesis of chitin by particulate fractions from Cunninghamella elegans. Microbios. In the Press.
    [Google Scholar]
  19. Porter C. A., Jaworski E. G. 1966; The synthesis of chitin by particulate preparations of Allomyces macrogynus. Biochemistry1149–1154
    [Google Scholar]
  20. Ruiz-herrera J., Bartnicki-Garcia S. 1974; Synthesis of cell wall microfibrils in vitro by a ‘soluble’ chitin synthetase from Mucor rouxii. Science; New York: 186357–359
    [Google Scholar]
  21. Trevelyan W. E., Harrison J. S. 1952; Studies in yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochemical Journal 50:298–303
    [Google Scholar]
  22. Ulane R. E., Cabib E. 1974; The activating system of chitin synthetase from Saccharomyces cerevisiae: purification and properties of an inhibitor of the activating factor. Journal of Biological Chemistry 249:3418–3422
    [Google Scholar]
  23. Vogel H. J. 1956; A convenient growth medium for Neurospora (medium N). Microbial Genetics Bulletin 13:42–43
    [Google Scholar]
  24. Yemm E. W., Willi A. J. 1954; Estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal 57:508–514
    [Google Scholar]
  25. Zechmeister L., Toth G. 1931; Zur Kenntnis der Hydrolysis von Chitin mit Salzsäure. Berichte der Deutschen chemischen Gesellschaft 64:2028–2031
    [Google Scholar]
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