1887

Abstract

Summary: Cytoplasmic ribosomal RNA extracted from and analysed by gel electrophoresis at 4 °C consisted of two components, with molecular weights (relative to rRNA) of 1·30 × 10 and 0·83 × 10 daltons, present in equimolar amounts. On heating briefly at 51 followed by rapid cooling, the 1·30 × 10 RNA completely dissociated into two components of molecular weights 0·70 × 10 and 0·56 × 10 (present in equimolar amounts). Fifty per cent dissociation of the molecule occurred at 28. That the integrity of the RNA molecule at low temperatures is maintained by its secondary structure was confirmed by electrophoresis under denaturing conditions (98%, v/v, formamide). To account for these phenomena, latent cleavage of the molecule is proposed.

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1976-03-01
2024-04-28
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References

  1. Applebaum S.W., Ebstein R.P., Wyatt G.R. 1966; Dissociation of ribosomal ribonucleic acid from silkmoth pupae by heat and dimethyl sulphoxide: evidence for specific cleavage points. Journal of Molecular Biology 21:29–41
    [Google Scholar]
  2. Atchison B.A., Bourque D.P., Wildman S.G. 1973; Preservation of 23s chloroplast RNA as a single chain of nucleotides. Biochimica et biophysica acta 331:382–389
    [Google Scholar]
  3. Barnard E.A. 1969; Ribonucleases. Annual Reviews of Biochemistry 38:677–732
    [Google Scholar]
  4. Bishop D.H.L., Claybrook J.R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373–387
    [Google Scholar]
  5. Borda L., Green M.H., Kamen M.D. 1969; Sedimentation properties of ribonucleic acid from Rhodopseudomonas spheroides.. Journal of General Microbiology 56:345–351
    [Google Scholar]
  6. Bostock C.J., Prescott D.M., Lauth M. 1971; Lability of 26s ribosomal RNA in Tetrahymena pyriformis.. Experimental Cell Research 66:260–262
    [Google Scholar]
  7. Cox R.A., Littauer U.Z. 1962; Ribonucleic acid from Escherichia coli. III. The influence of ionic strength and temperature on hydrodynamic and optical properties. Biochimica et biophysica acta 61:197–208
    [Google Scholar]
  8. Cross G.A.M. 1970; Sedimentation properties of polyribosomes, ribosomes and ribosomal subunits from Crithidia oncopelti. Biochimica et biophysica acta 204:470–477
    [Google Scholar]
  9. Dessev G.N., Grancharov K. 1973; Degradation of ribonucleic acid in rat liver ribosomes. Journal of Molecular Biology 76:425–444
    [Google Scholar]
  10. Greenberg J.R. 1969; Synthesis and properties of ribosomal RNA in Drosophila. Journal of Molecular Biology 46:85–98
    [Google Scholar]
  11. Grove B.K., Johnson T.C. 1973; The effect of ribonuclease on ribosomal RNA and subsequent polypeptide synthesis. Biochemical and Biophysical Research Communications 55:45–51
    [Google Scholar]
  12. Herskovits T.T. 1962; Nonaqueous solutions of DNA: factors determining the stability of the helical configuration in solution. Archives of Biochemistry and Biophysics 97:474–484
    [Google Scholar]
  13. Hüvös P., Gaál O., Vereczkey L. 1972; Effect of degradation of rat-liver ribosomal RNA on the structure of the ribosomal subunits. European Journal of Biochemistry 26:518–527
    [Google Scholar]
  14. Ingle J. 1968; Synthesis and stability of chloroplast ribosomal RNAs. Plant Physiology 43:1448–1454
    [Google Scholar]
  15. Ishikawa H. 1973; Primary and secondary nicks in the ribosomal ribonucleic acid of insects. Biochemical and Biophysical Research Communications 54:301–307
    [Google Scholar]
  16. Kirby K.S. 1965; Isolation and characterization of ribosomal ribonucleic acid. Biochemical Journal 96:266–269
    [Google Scholar]
  17. Leaver C.J. 1973; Molecular integrity of chloroplast ribosomal ribonucleic acid. Biochemical Journal 135:237–240
    [Google Scholar]
  18. Lessie T.S. 1965; The atypical ribosomal RNA complement of Rhodopseudomonas spheroides. Journal of General Microbiology 39:311–320
    [Google Scholar]
  19. Loening U.E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  20. Loening U.E. 1968a; Molecular weights cf ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355–365
    [Google Scholar]
  21. Loening U.E. 1968b; The fractionation of high molecular weight RNA. In Chromatographic and Electrophoretic Techniques 2, 2nd edn.. pp. 437–442 Smith I. Edited by London:: Heinemann Ltd;
    [Google Scholar]
  22. Newton B.A. 1957; Nutritional requirements and biosynthetic capabilities of the parasitic flagellate Strigomonas oncopelti. Journal of General Microbiology 17:708–717
    [Google Scholar]
  23. Parish J.H., Kirby K.S. 1966; Reagents which reduce interactions between ribosomal RNA and rapidly-labelled RNA from rat liver. Biochimica et biophysica acta 129:554–562
    [Google Scholar]
  24. Pene J.J., Knight E., Darnell J.E. 1968; Characterisation of a new low molecular weight RNA in Hela cell ribosomes. Journal of Molecular Biology 33:609–623
    [Google Scholar]
  25. Reijnders L., Sloof P., Sival J., Borst P. 1973; Gel electrophoresis of RNA under denaturing conditions. Biochimica et biophysica acta 324:320–333
    [Google Scholar]
  26. Shine J., Dalgarno L. 1973; Occurrence of heat-dissociable ribosomal RNA in insects: the presence of three polynucleotide chains in 26s RNA from cultured Aedes aegypti cells. Journal of Molecular Biology 75:57–72
    [Google Scholar]
  27. Shine J., Hunt J.A., Dalgarno L. 1974; Studies on the 3′-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with ‘hidden’ breaks in heat-dissociable insect 26s ribonucleic acid. Biochemical Journal 141:617–625
    [Google Scholar]
  28. Spencer R., Cross G.A.M. 1975; Purification and properties of nucleic acids from an unusual cytoplasmic organelle in the flagellate protozoan Crithidia oncopelti. Biochimica et biophysica acta 390:141–154
    [Google Scholar]
  29. Stanley W.M., Bock R.M. 1965; Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry 4:1302–1311
    [Google Scholar]
  30. Staynov D.Z., Pinder J.C., Gratzer W.B. 1972; Molecular weight determination of nucleic acids by gel electrophoresis in non-aqueous solution. Nature New Biology 235:108–110
    [Google Scholar]
  31. Stevens A.R., Pachler P.F. 1972; Discontinuity of 26s rRNA in Acanthamoeba castellani. Journal of Molecular Biology 66:225–237
    [Google Scholar]
  32. Szer W. 1969; Enzymic degradation of ribosomal RNA in isolated purified ribosomes. Biochemical and Biophysical Research Communications 35:653–658
    [Google Scholar]
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