1887

Abstract

Summary: Strand breaks accumulated in the DNA of a temperature-sensitive DNA ligase mutant of growing at the restrictive temperature, as detected by zone sedimentation through alkaline sucrose density gradients. The rate of strand breakage was increased by concomitant thymine starvation. Rifampicin and chloramphenicol inhibited the accumulation of strand breaks in the DNA. There was a correlation between the accumulation of strand breaks in the DNA and lethality, suggesting that such breaks are the basis for lethality at the restrictive temperature.

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/content/journal/micro/10.1099/00221287-94-2-297
1976-06-01
2024-04-27
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References

  1. Barner H. D., Cohen S. S. 1954; Induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli. Journal of Bacteriology 68:80–88
    [Google Scholar]
  2. Barner H. D., Cchen S. S. 1957; The isolation and properties of amino acid requiring mutants of a thymineless bacterium. Journal of Bacteriology 74:350–355
    [Google Scholar]
  3. Dean C., Pauling C. 1970; Properties of a deoxyribonucleic acid ligase mutant of Escherichia coli: X-ray sensitivity. Journal of Bacteriology 102:588–589
    [Google Scholar]
  4. Deutch C. E., Pauling C. 1971; Survival and macromolecular synthesis during incubation of Escherichia coli in limiting thymine. Journal of Bacteriology 106:197–203
    [Google Scholar]
  5. Goldmark P. J., Linn S. 1972; Purification and properties of the recBC DNase of Escherichia coli K-12. Journal of Biological Chemistry 247:1849–1860
    [Google Scholar]
  6. Hanawalt P. C. 1963; Involvement of synthesis of RNA in thymineless death. Nature; London: 198286
    [Google Scholar]
  7. Karu A. E., Mackay V., Goldmark P. J., Linn S. 1973; The recBC deoxyribonuclease of Escherichia coli K-12: substrate specificity and reaction intermediates. Journal of Biological Chemistry 248:4874–4884
    [Google Scholar]
  8. Kato T., Kondo S. 1970; Genetic and molecular characteristics of X-ray sensitive mutants of Escherichia coli defective in repair synthesis. Journal of Bacteriology 104:871–881
    [Google Scholar]
  9. Mcgrath R. A., Williams R. W. 1966; Reconstructionin vivo of irradiatedEscherichia coli deoxyribonucleic acid: the rejoining of broken pieces. Nature; London: 212534–535
    [Google Scholar]
  10. Nakayama H., Hanawalt P. 1975; Sedimentation analysis of deoxyribonucleic acid from thyminestarvedEscherichia coli. Journal of Bacteriology 121:537–547
    [Google Scholar]
  11. Pauling C., Beck L. A. 1975; Role of DNA ligase in the repair of single strand breaks induced in DNA by mild heating of Escherichia coli. Journal of General Microbiology 87:181–184
    [Google Scholar]
  12. Pauling C., Hamm L. 1968; Properties of a temperature sensitive radiation sensitive mutant ofEscherichia coli. Proceedings of the National Academy of Sciences of the United States of America 601495–1502
    [Google Scholar]
  13. Pauling C., Hamm L. 1969; Properties of a temperature-sensitive, radiation-sensitive mutant ofEscherichia coli. II. DNA replication. Proceedings of the National Academy of Sciences of the United States of America 641195–1202
    [Google Scholar]
  14. Pauling C., Hanawalt P. 1965; Nonconservative DNA replication in bacteria after thymine starvation. Proceedings of the National Academy of Sciences of the United States of America 541728–1735
    [Google Scholar]
  15. Reichenbach D. L., Schaiberger G. E., Sallman B. 1971; The effect of thymine starvation on chromosomal structure ofEscherichia coli JG-151. Biochemical and Biophysical Research Communications 42:23–29
    [Google Scholar]
  16. Sedgwick S. G., Bridges B. R. 1972; Evidence for indirect production of DNA strand scissions during mild heating ofEscherichia coli. Journal of General Microbiology 71:191–193
    [Google Scholar]
  17. Walker J. R. 1970; Thymine starvation and single breaks in chromosomal deoxyribonucleic acid ofEscherichia coli. Journal of Bacteriology 104:1391–1392
    [Google Scholar]
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