1887

Abstract

Summary: Liquid batch cultures of showed a typical growth pattern coupled with a rapid fall in the pH of the medium followed by a rise in pH after the cessation of growth. Production of conidia was associated with this final pH rise. In glucose-limited continuous cultures without pH control, a low pH was maintained and the organism remained totally vegetative. Adjusting the pH to values above 5·0 for 6 h or more led to maximum production of conidia. The rate of development of conidia was independent of the growth rate but the rate of production of conidia was greatest at high growth rates. Glucose inhibited both production of conidia and the rate of development of conidia in phosphate-limited and nitrogen-limited cultures. The increased rates of conidium production at high growth rates were achieved not by the greater proliferation of apices, but by the development of a greater proportion of the apices into conidia.

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1977-03-01
2024-05-02
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References

  1. Bainbridge B.W., Bull A.T., Pirt S.J., Rowley B.I., Trinci A.P.J. 1971; Biochemical and structural changes in non-growing maintained and autolysing cultures of Aspergillus nidulans. Transactions of the British Mycological Society 56:371–385
    [Google Scholar]
  2. Carter B.L.A., Bull A.T. 1969; Studies of fungal growth and intermediary carbon metabolism under steady and non steady state conditions. Biotechnology and Bioengineering 11:785–804
    [Google Scholar]
  3. Carter B.L.A., Bull A.T. 1971; The effect of oxygen tension in the medium on the morphology and growth kinetics of Aspergillus nidulans. Journal of General Microbiology 65:265–273
    [Google Scholar]
  4. Carter B.L.A., Bull A.T., Pirt S.J., Rowley B.I. 1971; Relationship between energy substrate utilisation and specific growth rate in Aspergillus nidulans. Journal of Bacteriology 108:309–313
    [Google Scholar]
  5. Dygert S., Li L.H., Florida D., Thoma J.A. 1965; Determination of reducing substances with improved precision. Analytical Biochemistry 13:367–374
    [Google Scholar]
  6. Fairbairn N.J. 1953; A modified anthrone reagent. Chemistry and Industry 72:86
    [Google Scholar]
  7. Fiddy C., Trinci A.P.J. 1975; Kinetics and morphology of glucose-limited cultures of moulds grown in a chemostat and on solid media. Archives of Microbiology 103:191–197
    [Google Scholar]
  8. Marchant R. 1975; An ultrastructural study of ‘phialospore’ formation in Fusarium culmorum grown in continuous culture. Canadian Journal of Botany 53:1978–1987
    [Google Scholar]
  9. Marchant R., White M.F. 1966; Spore swelling and germination in Fusarium culmorum. Journal of General Microbiology 42:237–244
    [Google Scholar]
  10. Marchant R., White M.F. 1967; The carbon metabolism and swelling of Fusarium culmorum conidia. Journal of General Microbiology 48:65–77
    [Google Scholar]
  11. Morton A.G. 1967; Morphogenesis in fungi. Science Progress 55:597–611
    [Google Scholar]
  12. Ng A.M.L., Smith J.E., Mcintosh A.F. 1973; Conidiation of Aspergillu snigerin continuous culture. Archiv für Mikrobiologie 88:119–126
    [Google Scholar]
  13. Righelato R.C., Trinci A.P.J., Pirt S.J., Peat A. 1968; The influence of maintenance energy and growth rate on the metabolic activity, morpholog> and conidiation of Penicillium chrysogenum. Journal of General Microbiology 50:399–412
    [Google Scholar]
  14. Robinson P.M., Smith J.M. 1976; Morphogenesis and growth kinetics of Geotrichum candidum in continuous culture. Transactions of the British Mycological Society 66:413–420
    [Google Scholar]
  15. Rowley B.I., Pirt S.J. 1972; Melanin production by Aspergillus nidulans in batch and chemostat cultures. Journal of General Microbiology 72:553–563
    [Google Scholar]
  16. Solomons G.L. 1972; Improvements in the design and operation of the chemostat. In 5th International Symposium on Continuous Culture of Micro-organisms Oxford pp. 217–228 Dean A.C.R., Pirt S.J., Tempest D.W. Edited by London: Academic Press;
    [Google Scholar]
  17. Steensland H. 1973; Continuous culture of a sewage fungus Fusarium aquaeductuum. Archiv für Mikrobiologie 93:287–294
    [Google Scholar]
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