1887

Abstract

Summary: , a mycoplasma-like organism, was grown at 56 °C and pH 2. The microfluidity of membrane vesicles was determined for the temperature range −2 to 35 °C using spin labels. The temperature at which the low-temperature phase transition occurred in membrane lipids increased with increasing Ca concentration. However, there was no evidence of a low-temperature lipid phase transition for membrane vesicles suspended in a medium containing monovalent instead of bivalent cations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-99-2-379
1977-04-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/99/2/mic-99-2-379.html?itemId=/content/journal/micro/10.1099/00221287-99-2-379&mimeType=html&fmt=ahah

References

  1. Bruni A., Van Duck P.W.M., De Gier J. 1975; The role of phospholipid acyl chains in the activation of mitochondrial ATPase complex. Biochimica et biophysica Acta 406:315–328
    [Google Scholar]
  2. Chapman D. 1975; Phase transitions and fluidity characteristics of lipids and cell membranes. Quarterly Reviews of Biophysics 8:185–235
    [Google Scholar]
  3. Darland D., Brock T.D., Samsonoff W., Conti S. 1970; A thermophilic acidophilic mycoplasma isolated from a coal refuse pile. Science 8:1416–1418
    [Google Scholar]
  4. Davis D.G., Inesi G., Gulik-Krzywicki T. 1976; Lipid molecular motion and enzyme activity in sarcoplasmic reticulum membranes. Biochemistry 15:1271–1276
    [Google Scholar]
  5. Esfahani M., Limbrik A.R., Knutton S., Oka T., Wakil S.J. 1971; The molecular organization of lipids in the membrane of E. coli. Proceedings of the National Academy of Sciences of the United States of America 68:3180–3184
    [Google Scholar]
  6. Griffith O.H., Jost P.C. 1976; Lipid spin labels in biological membranes. In Spin Labeling Theory and Applications pp. 453–523 Berliner L.J. Edited by New York: Academic Press.;
    [Google Scholar]
  7. Jacobson K., Papahadjopoulos D. 1975; Phase transitions and phase separations induced by changes in temperature, pH, and concentration of bivalent cations. Biochemistry 14:152–161
    [Google Scholar]
  8. Kimelberg H.K., Papahadjopoulos D. 1974; Effect of phospholipid acyl chain fluidity, phase transitions and cholesterol on (Na+ + K+)-stimulated ATPase. Journal of Biological Chemistry 249:1071–1080
    [Google Scholar]
  9. Ledebo I. 1976; Divalent cations in the envelope of a psychrophilic Achromobacter. Journal of General Microbiology 94:351–358
    [Google Scholar]
  10. Lee A.G. 1975; Functional properties of biological membranes: a physico-chemical approach. Progress in Biophysics and Molecular Biology 29:3–56
    [Google Scholar]
  11. Linden C.D., Wright K.L., McConnell H.M., Fox C.F. 1973; Lateral phase separations in membrane lipids and the mechanism of sugar transport in E. coli. Proceedings of the National Academy of Sciences of the United States of America 70:2271–2275
    [Google Scholar]
  12. Lis L.J., Kauffman J.W., Shriver D.F. 1975; Effect of ions on phospholipid layer structure as indicated by Raman spectroscopy. Biochimica et biophysica acta 406:453–464
    [Google Scholar]
  13. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Ruwart M.J., Haug A. 1975; Membrane properties of Thermoplasma acidophila. Biochemistry 14:860–866
    [Google Scholar]
  15. Shimshick E.J., McConnell H.M. 1973; Lateral phase separation in phospholipid membranes. Biochemistry 12:2351–2360
    [Google Scholar]
  16. Smith G.G., Ruwart M.J., Haug A. 1974; Lipid phase transitions in membrane vesicles from Thermoplasma acidophila. FEBS Letters 45:96–98
    [Google Scholar]
  17. Stahl S., Ljunger C. 1976; Calcium uptake by Bacillus stearothermophilus: a requirement for thermophilic growth. FEBS Letters 63:184–187
    [Google Scholar]
  18. Träuble H., Eibl H. 1975; Molecular interactions in lipid bilayers. In Functional Linkage in Biomolecular Systems pp. 59–101 Schmitt F.O, Schneider D.M, Crothers D.M. Edited by New York: Raven Press.;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-99-2-379
Loading
/content/journal/micro/10.1099/00221287-99-2-379
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error