f Isolation and characterization of a strain of Rhodobacter sulfidophilus: A bacterium which grows autotrophically with dimethylsulphide as electron donor
- Authors: Steven P. Hanlon, Robert A. Holt, Geoffrey R. Moore, Alastair G. McEwan†
- Author for correspondence: Alastair G. McEwan. Tel: +61 7 365 4878. Fax: +61 7 365 4620. e-mail firstname.lastname@example.org
- Microbiology, August 1994 140: 1953-1958, doi: 10.1099/13500872-140-8-1953
- Subject: Physiology And Growth
- Published Online:
A marine photosynthetic bacterium (strain SH1) was isolated after enrichment under phototrophic conditions in media containing dimethylsulphide (DMS) and bicarbonate (HCO− 3) as potential carbon sources. Analysis of culture medium using nuclear magnetic resonance spectrometry showed that during phototrophic and chemotrophic growth of strain SH1 on DMS/HCO− 3 dimethylsulphoxide (DMSO) was produced from DMS. These results indicate that strain SH1 grew autotrophically with DMS serving as an electron donor in photosynthesis and respiration, but not as a carbon source. Biochemical characterization and 16S rRNA analysis indicated that the isolate was a strain of Rhodobacter sulfidophilus. An assay for the enzyme catalysing the oxidation of DMS (DMS:acceptor oxidoreductase) was developed by measuring electron transfer from DMS to 2,6-dichlorophenolindophenol (DCPIP). This reaction was dependent on phenazine ethosulphate to mediate electron transfer from DMS:acceptor oxidoreductase to DCPIP. DMS:acceptor oxidoreductase was found to have a periplasmic location in strain SH1 as was a reduced methylviologen: DMSO oxidoreductase activity. Zymogram staining patterns of periplasmic fractions indicated that DMS: acceptor oxidoreductase and DMSO reductase were distinct enzymes. This was confirmed by resolution of the two activities by gel filtration.
Present address: Department of Microbiology, University of Queensland, Brisbane, Qld 4072, Australia.
© Society for General Microbiology, 1994 | Published by the Microbiology Society
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