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Volume 141, Issue 10

Molecular analysis of an extracellular protease gene from Free

Abstract

Summary: The structural gene encoding the extracellular protease of strain 93 was cloned in and sequenced. The cloned DNA fragment contained a 1761 bp ORF encoding a 587 amino acid protein. The deduced polypeptide is composed of a 25 amino acid signal peptide and a 562 amino acid extracellular polypeptide with a calculated molecular mass of 63 156 Da. Protease analysis using a gelatin-containing SDS-polyacrylamide gel detected the presence of a 62 kDa protease that was present in the culture supernatant fractions of the wild-type strain and of bearing a pUC119 recombinant with the DNA insert. The protease activity was inhibited by zinc- and metal-specific inhibitors such as EDTA and 1,10-phenanthroline, which suggested that it is a metalloprotease. The deduced amino acid sequence of PrtVp has 32% identity with that of the collagenase of , but has no identity with those of the bacterial proteases. A conserved zinc-binding domain was also found in PrtVp from homology comparison with other metalloproteases. This PrtVp can cause weak haemolysis on blood agar.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): cloning , protease gene and sequence. Vibrio parahaemolyticus
© Society for General Microbiology 1995
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1995-10-01
2024-04-28
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Molecular analysis of an extracellular protease gene from Vibrio parahaemolyticus
Microbiology 141, 2569 (1995); https://doi.org/10.1099/13500872-141-10-2569
/content/journal/micro/10.1099/13500872-141-10-2569
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