f A group I intron in the terminase gene of Lactobacillus delbrueckii subsp. lactis phage LL-H
- Authors: Merja Mikkonen, Tapani Alatossava
- Tel: +358 81 553 1792. Fax: +358 81 553 1799. e-mail: Merja.Mikkonen@csc.fi
- Microbiology, September 1995 141: 2183-2190, doi: 10.1099/13500872-141-9-2183
- Subject: Genetics And Molecular Biology
- Published Online:
An 837 nt long group IA intron was discovered in the Lactobacillus delbrueckii subsp. lactis virulent phage LL-H genome. The LL-H intron conforms well to the secondary structure that is common to all group I introns. The only exception is that the extreme 3′ nucleotide of the intron is an A residue instead of the usual G; despite this the intron is efficiently spliced in vivo. This LL-H intron contains an ORF, ORF168, which shows homology with endonucleases encoded by ORFs contained in Bacillus subtilis phage introns. At present, the LL-H intron is the only one found in the phages of lactic acid bacteria and the first one to be found in a phage belonging to the most abundant taxonomic group, group B or Siphoviridae. The LL-H intron interrupts gene terL, the product of which (50.5 kDa, TerL) is significantly homologous to the large subunit of B. subtilis phage SPP1 terminase. The product of the upstream gene, terS of LL-H (15.9 kDa, TerS), shows homology to small subunits of B. subtilis phage terminases.
- Keyword(s): phage evolution, RNA structure, terminase, endonuclease, group I intron, lactic acid bacteria
© Society for General Microbiology 1995 | Published by the Microbiology Society
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