f gdhB, a gene encoding a second quinoprotein glucose dehydrogenase in Pantoea citrea, is required for pink disease of pineapple
- Authors: Catherine J. Pujol†, Clarence I. Kado
- Author for correspondence: Clarence I. Kado. Tel: + 1 530 752 0325. Fax: + 1 530 752 5674. e-mail: firstname.lastname@example.org
- First Published Online: 01 May 1999, Microbiology 145: 1217-1226, doi: 10.1099/13500872-145-5-1217
- Subject: Genetics And Molecular Biology
- Issue Published:
The pink disease of pineapple, caused by the bacterium Pantoea citrea, is characterized by a dark coloration on fruit slices after canning. A glucose dehydrogenase (Gdh) encoded by the gdhA gene has been implicated in the colour formation activity of P. citrea. In this paper it has been shown that P. citrea contains a second, homologous gdh gene and its product, GdhB, represents the main source of Gdh activity in this organism. Unlike gdhA, gdhB is constitutively expressed during the exponential phase of growth and is induced in stationary phase. A previously isolated chemical mutant, CMC6, which is deficient in Gdh activity and pink disease formation, failed to express gdhB during the stationary phase of growth. The CMC6 mutant can be complemented by a 54 bp DNA fragment located upstream of gdhA. This fragment, which contains an operator-like 11 bp inverted repeat, strongly enhances the expression of gdhA, probably by titrating away a negative effector of its expression. These results illustrate the complex interplay operating between the two gdh genes and emphasize the role of glucose metabolism in the pathway leading to pink disease.
Present address: Medical Pathology Department, UCD Medical Center, Room 3300A, 4645 2nd Avenue, Sacramento, CA 95817, USA
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