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Volume 170, Issue 3

Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria Open Access

Abstract

Sialic acid (Sia) transporters are critical to the capacity of host-associated bacteria to utilise Sia for growth and/or cell surface modification. While N-acetyl-neuraminic acid (Neu5Ac)-specific transporters have been studied extensively, little is known on transporters dedicated to anhydro-Sia forms such as 2,7-anhydro-Neu5Ac (2,7-AN) or 2,3-dehydro-2-deoxy-Neu5Ac (Neu5Ac2en). Here, we used a Sia-transport-null strain of to investigate the function of members of anhydro-Sia transporter families previously identified by computational studies. First, we showed that the transporter NanG, from the Glycoside-Pentoside-Hexuronide:cation symporter family, is a specific 2,7-AN transporter, and identified by mutagenesis a crucial functional residue within the putative substrate-binding site. We then demonstrated that NanX transporters, of the Major Facilitator Superfamily, also only transport 2,7-AN and not Neu5Ac2en nor Neu5Ac. Finally, we provided evidence that SiaX transporters, of the Sodium-Solute Symporter superfamily, are promiscuous Neu5Ac/Neu5Ac2en transporters able to acquire either substrate equally well. The characterisation of anhydro-Sia transporters expands our current understanding of prokaryotic Sia metabolism within host-associated microbial communities.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacteria , microbiome , mucosa , sialic acid , sialidase and transporter
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/X011054/1)
    • Principle Award Recipient: NathalieJuge
  • Biotechnology and Biological Sciences Research Council (Award BB/X011011/1)
    • Principle Award Recipient: NathalieJuge
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-03-15
2024-04-27
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Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria
Microbiology 170, 001448 (2024); https://doi.org/10.1099/mic.0.001448
/content/journal/micro/10.1099/mic.0.001448
/content/journal/micro/10.1099/mic.0.001448
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