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Volume 170, Issue 3

A multi-colour fluorogenic tag and its application in Open Access

Abstract

Fluorescent proteins (FPs) have always been a crucial part of molecular research in life sciences, including the research into the human fungal pathogen but have obvious shortcomings such as their relatively large size and long maturation time. However, the next generation of FPs overcome these issues and rely on the binding of a fluorogen for the protein to become fluorescently active. This generation of FPs includes the improved version of Fluorescence activating and Absorption Shifting Tag (iFAST). The binding between the fluorogen and the iFAST protein is reversible, thus resulting in reversible fluorescence. The fluorogens of iFAST are analogues of 4-hydroxylbenzylidene-rhodanine (HBR). These HBR analogues differ in spectral properties depending on functional group substitutions, which gives the iFAST system flexibility in terms of absorbance and emission maxima. In this work we describe and illustrate the application of iFAST as a protein tag and its reversible multi-colour characteristics in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Candida albicans , fluorogenic tag , iFAST and microscopy
Funding
This study was supported by the:
  • Onderzoeksraad, KU Leuven (Award C14/22/075)
    • Principle Award Recipient: Van DijckPatrick
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2024-03-27
2024-04-27
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A multi-colour fluorogenic tag and its application in Candida albicans
Microbiology 170, 001451 (2024); https://doi.org/10.1099/mic.0.001451
/content/journal/micro/10.1099/mic.0.001451
/content/journal/micro/10.1099/mic.0.001451
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