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Volume 155, Issue 11

Differential effects of short-chain fatty acids and iron on expression of in Shiga-toxigenic Free

Abstract

Shiga-toxigenic (STEC) colonizing the bowel are exposed to a variety of short-chain fatty acids (SCFAs), including acetate, propionate and butyrate, produced by gut microflora. However, the total concentrations and relative amounts of SCFAs in the lumen vary with intestinal niche. Here we report that conditions simulating SCFA concentrations present in the human gut trigger expression of the gene, which encodes an adherence-conferring outer-membrane protein of pathogenic . We show that growth under conditions simulating colonic, but not ileal, SCFA concentrations increases expression in three tested STEC strains, with the strongest expression detected in LEE-negative STEC O113:H21 strain 98NK2. Expression of is known to be subject to Fur-mediated iron repression in O157:H7 STEC, and the same occurs in 98NK2. However, exogenous iron did not repress expression in the presence of colonic SCFAs in either 98NK2 or the O157:H7 strain EDL933. Moreover, exposure to the iron chelator 2,2′-dipyridyl caused no further enhancement of expression over that induced by colonic SCFAs. These findings indicate that SCFAs regulate expression in STEC independently of iron. Increased expression of under colonic but not ileal SCFA conditions possibly may contribute to preferential colonization of the human colon by STEC.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): HUS, haemolytic uraemic syndrome , LEE, locus of enterocyte effacement , SCFA, short-chain fatty acid , STEC, Shiga-toxigenic Escherichia coli and UPEC, uropathogenic E. coli
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2009-11-01
2024-04-27
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Differential effects of short-chain fatty acids and iron on expression of iha in Shiga-toxigenic Escherichia coli
Microbiology 155, 3554 (2009); https://doi.org/10.1099/mic.0.029454-0
/content/journal/micro/10.1099/mic.0.029454-0
/content/journal/micro/10.1099/mic.0.029454-0
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