1887

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Volume 158, Issue 7

YidC1 and YidC2 are functionally distinct proteins involved in protein secretion, biofilm formation and cariogenicity of Free

Abstract

The cariogenic bacterium has two paralogues of the YidC/Oxa1/Alb3 family of membrane protein insertases/chaperones. Disruption of results in loss of genetic competence, decreased membrane-associated ATPase activity and stress sensitivity (acid, osmotic and oxidative). Elimination of has less severe effects, with little observable effect on growth or stress sensitivity. To examine the respective roles of YidC1 and YidC2, a conditional expression system was developed allowing simultaneous elimination of both endogenous YidCs. The function of the YidC C-terminal tails was also investigated and a chimeric YidC1 protein appended with the C terminus of YidC2 enabled YidC1 to complement a Δ mutant for stress tolerance, ATP hydrolysis activity and extracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. Elimination of or affected levels of extracellular proteins, including GtfB, GtfC and adhesin P1 (AgI/II, PAc), which were increased without YidC1 but decreased in the absence of YidC2. Both and were shown to contribute to biofilm formation and to cariogenicity in a rat model. Collectively, these results provide evidence that YidC1 and YidC2 contribute to cell surface biogenesis and protein secretion in and that differences in stress sensitivity between the Δ and Δ mutants stem from a functional difference in the C-termini of these two proteins.

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2012-07-01
2024-04-27
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YidC1 and YidC2 are functionally distinct proteins involved in protein secretion, biofilm formation and cariogenicity of Streptococcus mutans
Microbiology 158, 1702 (2012); https://doi.org/10.1099/mic.0.059139-0
/content/journal/micro/10.1099/mic.0.059139-0
/content/journal/micro/10.1099/mic.0.059139-0
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