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Volume 158, Issue 11

Slr0643, an S2P homologue, is essential for acid acclimation in the cyanobacterium sp. PCC 6803 Free

Abstract

Every cyanobacterial species contains genes encoding site-2-protease (S2P) homologues. The studied prokaryotic S2P homologues play essential roles in regulating stress responses through intramembrane proteolysis of membrane-bound anti-sigma factors. Here, the gene encoding Slr0643, one of four S2P homologues in sp. PCC 6803, was insertionally disrupted to explore its physiological role. Only a partially segregated mutant was obtained, indicating the essentiality of the gene product for growth. A pivotal role of fully functional Slr0643 in acid acclimation was demonstrated by defective acid acclimation to pH 6.5 in the mutant and transient induction of in the wild-type after transfer from pH 7.5 to 6.5. DNA microarray and quantitative RT-PCR analyses of mutant and wild-type strains at pH 7.5 versus pH 6.5 identified genes involved in early acid acclimation and revealed genes expressed differentially due to disruption. Early acid acclimation to pH 6.5 in the wild-type strain included upregulation of , and and downregulation of and , as well as downregulation of porins and upregulation of inorganic carbon and nitrogen transporters. The inability of the mutant strain to survive at pH 6.5 was found to be related to defective photosynthesis and excess expression of NADH dehydrogenase, together with excessive upregulation of carbon transporter and repression of nitrogen transporter and metabolism genes. Most interestingly, analysis of microarray data revealed the close relationship between disruption and expression of the operon. Thus it is suggested that Slr0643/Sll0857/SigH might act through an S2P/anti-Sigma factor/Sigma factor mechanism to play a role in acid acclimation.

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  • Accepted:
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  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (NSFC)
  • Fundamental Research Funds for the Central Universities (Award SCUT 2009ZM0006, 31270085 and 30800609)
  • NSFC Project (Award 20876058)
  • Major State Basic Research Development Program of China (Award 2011CB200901 and 2011CB200904 )
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2012-11-01
2024-04-28
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Slr0643, an S2P homologue, is essential for acid acclimation in the cyanobacterium Synechocystis sp. PCC 6803
Microbiology 158, 2765 (2012); https://doi.org/10.1099/mic.0.060632-0
/content/journal/micro/10.1099/mic.0.060632-0
/content/journal/micro/10.1099/mic.0.060632-0
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