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Volume 160, Issue 10

Global regulator Anr represses PlcH phospholipase activity in when oxygen is limiting Free

Abstract

Haemolytic phospholipase C (PlcH) is a potent virulence and colonization factor that is expressed at high levels by within the mammalian host. The phosphorylcholine liberated from phosphatidylcholine and sphingomyelin by PlcH is further catabolized into molecules that both support growth and further induce expression. We have shown previously that the catabolism of PlcH-released choline leads to increased activity of Anr, a global transcriptional regulator that promotes biofilm formation and virulence. Here, we demonstrated the presence of a negative feedback loop in which Anr repressed transcription and we proposed that this regulation allowed for PlcH levels to be maintained in a way that promotes productive host–pathogen interactions. Evidence for Anr-mediated regulation of PlcH came from data showing that growth at low oxygen (1 %) repressed PlcH abundance and transcription in the WT, and that transcription was enhanced in an Δ mutant. The promoter featured an Anr consensus sequence that was conserved across all genomes and mutation of conserved nucleotides within the Anr consensus sequence increased expression under hypoxic conditions. The Anr-regulated transcription factor Dnr was not required for this effect. The loss of Anr was not sufficient to completely derepress transcription as GbdR, a positive regulator of , was required for expression. Overexpression of Anr was sufficient to repress transcription even at 21 % oxygen. Anr repressed expression and phospholipase C activity in a cell culture model for –epithelial cell interactions.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • National Institutes of Health (Award RO1A109170)
  • NRSA Institutional Research Training Grant Fellowship (Award T32-DK007301)
  • National Institute of General Medical Sciences (Award P30GM106394)
  • Cystic Fibrosis Foundation Research Development Program (Award STANTO07R0)
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2014-10-01
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Global regulator Anr represses PlcH phospholipase activity in Pseudomonas aeruginosa when oxygen is limiting
Microbiology 160, 2215 (2014); https://doi.org/10.1099/mic.0.081158-0
/content/journal/micro/10.1099/mic.0.081158-0
/content/journal/micro/10.1099/mic.0.081158-0
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