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Volume 153, Issue 12

Molecular characterization of the copper transport system in Free

Abstract

The gene codes for a putative copper-translocating P-type ATPase and the downstream gene codes for a copper chaperone. Genome database analyses demonstrate that these copper transport genes are highly conserved in . The expression of and was inducible by copper and to some extent by ferric and lead ions. A mutant strain containing a partially deleted gene was more sensitive than the parent strain to copper, ferric and lead ions. The copper-sensitive phenotype was due to the accumulation of intracellular copper and thus the product is involved in the export of copper ions. The metal-sensitive phenotype of the mutant was complemented by a 2.7 kbp DNA containing . We have cloned and overexpressed the metal-binding domains of CopA and CopZ and have shown by site-directed mutagenesis that the cysteine residues in the CXXC metal-binding motif in CopA are involved in copper binding and thus play an important role in copper transport in .

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Keyword(s): BCA, bicinchoninic acid , IAA, iminodiacetic acid-agarose and MBD, metal-binding domain
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2007-12-01
2024-05-10
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Molecular characterization of the copper transport system in Staphylococcus aureus
Microbiology 153, 4274 (2007); https://doi.org/10.1099/mic.0.2007/009860-0
/content/journal/micro/10.1099/mic.0.2007/009860-0
/content/journal/micro/10.1099/mic.0.2007/009860-0
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