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Volume 154, Issue 3

Regulation of the gene encoding the major polygalacturonase of by Clp and RpfF Free

Abstract

Exopolysaccharide and several extracellular enzymes of pv. campestris (Xcc), the causative agent of black rot in crucifers, are virulence factors. In this study, sequence and mutational analysis has demonstrated that Xcc encodes the major polygalacturonase, a member of family 28 of the glycosyl hydrolases. Using the 5′ RACE (rapid amplification of cDNA ends) method, the transcription initiation site was mapped at 102 nt downstream of a Clp (cAMP receptor protein-like protein)-binding site. Transcriptional fusion assays showed that transcription is greatly induced by polygalacturonic acid, positively regulated by Clp and RpfF (an enoyl-CoA hydratase homologue which is required for the synthesis of -11-methyl-2-dodecenoic acid, a low-molecular-mass diffusible signal factor), subjected to catabolite repression, which is independent of Clp or RpfF, and repressed under conditions of oxygen limitation or nitrogen starvation. Our findings extend previous work on Clp and RpfF regulation to show that they both influence the expression of in Xcc.

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Keyword(s): 5′ RACE, rapid amplification of cDNA ends , AUAP, abridged universal amplification primer , CBS, Clp-binding site , DSF, diffusible signal factor , PGA, polygalacturonic acid , TIS, transcription initiation site and Xcc, Xanthomonas campestris pv. campestris
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2008-03-01
2024-05-10
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Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF
Microbiology 154, 705 (2008); https://doi.org/10.1099/mic.0.2007/012930-0
/content/journal/micro/10.1099/mic.0.2007/012930-0
/content/journal/micro/10.1099/mic.0.2007/012930-0
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