1887

Abstract

The haem pigment of is composed of -oxo bishaem, [Fe(III)PPIX]O, but the nature of that generated by species has not been established. Mössbauer, Raman and UV-visible spectrophotometry were used to characterize the haem pigment of and . Mössbauer and Raman spectroscopy revealed the major haem species to be monomeric iron protoporphyrin IX, Fe(III)PPIX.OH (haematin). The terminal growth pH of both species on blood agar was between 5·8 and 6·0, which favours the formation and maintenance of monomeric Fe(III)PPIX.OH. Incubation of and with oxyhaemoglobin at pH 6·5 resulted in formation of aquomethaemoglobin which was degraded to generate Fe(III)PPIX.OH which in turn became cell-associated, whilst incubation at pH 7·5 resulted in formation of [Fe(III)PPIX]O. It is concluded that both species degrade oxyhaemoglobin to form [Fe(III)PPIX]O as an intermediate, which is converted to Fe(III)PPIX.OH through a depression in pH. The low pH encourages cell-surface deposition of insoluble Fe(III)PPIX.OH which would act as a barrier against oxygen and reactive oxygen species, and also protect against HO through its inherent catalase activity.

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2003-07-01
2024-04-26
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