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Volume 152, Issue 4

Exploitation of a -lactamase reporter gene fusion in the carbapenem antibiotic production operon to study adaptive evolution in Free

Abstract

subsp. strain ATTn10 produces the -lactam antibiotic 1-carbapen-2-em-3-carboxylic acid (carbapenem) by expressing the operon. Mutants exhibiting increased carbapenem gene transcription were positively selected using an engineered strain with a functional -lactamase translational fusion in , the last gene of the operon. However, spontaneous ampicillin-resistant mutants were isolated even when transcription of  : :  was blocked by a strongly polar mutation in . The mechanism of resistance was shown to be due to cryptic IS elements transposing upstream of  : : , thereby providing new promoters enabling  : :  transcription. Southern blots showed that IS was present in multicopy in ATTn10. In addition, a Tn genetic remnant was discovered. The results offer insights into the genetic archaeology of strain ATTn10 and highlight the powerful impacts of cryptic IS elements in bacterial adaptive evolution.

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  • Accepted:
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  • Published Online:
Keyword(s): Ap, ampicillin , Car, 1-carbapen-2-em-3-carboxylic acid , Ecc, Erwinia carotovora subspecies carotovora and Tc, tetracycline
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2006-04-01
2024-04-26
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Exploitation of a β-lactamase reporter gene fusion in the carbapenem antibiotic production operon to study adaptive evolution in Erwinia carotovora
Microbiology 152, 1089 (2006); https://doi.org/10.1099/mic.0.28575-0
/content/journal/micro/10.1099/mic.0.28575-0
/content/journal/micro/10.1099/mic.0.28575-0
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