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Volume 153, Issue 1

An -like quorum-sensing system negatively regulates pyoluteorin production in sp. M18 Free

Abstract

sp. M18, isolated from the watermelon rhizosphere, is antagonistic against a number of soil-borne pathogens. This strain produces an uncharacterized red pigment, pyoluteorin (Plt), and two -acylhomoserine lactones (AHLs). A previously isolated red-pigment-defective mutant, M18-T510, contains an insert within a gene similar to in PAO1. The M18 gene product is responsible for the production of two AHL signals: -butyryl-homoserine lactone and -hexanoylhomoserine lactone. Mutants defective in either or showed enhanced Plt biosynthesis due to loss of transcriptional repression, which was mediated, at least in part, by suppressed expression of the activator PltR. A Plt-specific ABC transporter was also upregulated in the mutants in a Plt-dependent manner. In comparison with the wild-type strain, the mutants survived longer during stationary-phase growth.

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Keyword(s): AHL, N-acylhomoserine lactone , BHL, N-butyrylhomoserine lactone , CT, threshold cycle , HHL, N-hexanoylhomoserine lactone , PCA, phenazine-1-carboxylic acid , Plt, pyoluteorin and QS, quorum sensing
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2007-01-01
2024-04-28
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An rhl-like quorum-sensing system negatively regulates pyoluteorin production in Pseudomonas sp. M18
Microbiology 153, 16 (2007); https://doi.org/10.1099/mic.0.29211-0
/content/journal/micro/10.1099/mic.0.29211-0
/content/journal/micro/10.1099/mic.0.29211-0
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