1887

Abstract

is a major pathogen of humans throughout the world. Using commercial mAbs to capsular epitopes, strains of manifest five distinct serotypes – A, B, C, D and AD. Previous studies demonstrated significant divergence among serotypes A, B, C and D, which are thought to be haploid. In this study the origins and evolution of strains of serotype AD were investigated. A portion (537 bp) of the laccase gene was cloned and sequenced from 14 strains of serotype AD. Each strain contained two different alleles and sequences for both alleles were obtained. These sequences were compared to those from serotypes A, B, C and D. This analysis indicated that each of the 14 serotype AD strains contained two phylogenetically distinct haplotypes: one haplotype was highly similar to the serotype A group and the other to the serotype D group. To explain the origins of these serotype AD strains, genealogical analysis is consistent with at least three recent and independent hybridization events. The results demonstrate that the evolution of is continuing and dynamic.

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2002-01-01
2024-04-26
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References

  1. Aulakh H. S., Straus S. E., Kwon-Chung K. J. 1981; Genetic relatedness of Filobasidiella neoformans ( Cryptococcus neoformans ) and Filobasidiella bacillispora ( Cryptococcus bacillispora ) as determined by DNA base composition and sequence homology studies. Int J Syst Bacteriol 31:97–103 [CrossRef]
    [Google Scholar]
  2. Boekhout T., Theelen B., Diaz M., Fell J. W., Hop W. C. J., Abeln E. C. A., Dromer F., Meyer W. 2001; Hybrid genotypes in the pathogenic yeast Cryptococcus neoformans . Microbiology 147:891–907
    [Google Scholar]
  3. Brandt M. E., Bragg S. L., Pinner R. W. 1993; Multilocus enzyme typing of Cryptococcus neoformans . J Clin Microbiol 31:2819–2823
    [Google Scholar]
  4. Brandt M. E., Hutwagner L. C., Kuykendall R. J., Pinner R. W. 1995; Comparison of multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for molecular subtyping of Cryptococcus neoformans . J Clin Microbiol 33:1890–1895
    [Google Scholar]
  5. Brandt M. E., Hutwagner L. C., Klug L. A. 9 other authors 1996; Molecular subtype distribution of Cryptococcus neoformans in four areas of the United States. J Clin Microbiol 34:912–917
    [Google Scholar]
  6. Casadevall A., Perfect J. R. 1998 Cryptococcus neoformans Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: A maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  8. Franzot S. P., Salkin I. F., Casadevall A. 1999; Cryptococcus neoformans var. grubii : separate varietal status for Cryptococcus neoformans serotype A isolates. J Clin Microbiol 37:838–840
    [Google Scholar]
  9. Kabasawa K., Itagaki H., Ikeda R., Shinoda T., Kagaya K., Fukazawa Y. 1991; Evaluation of a new method for identification of Cryptococcus neoformans which uses serologic tests aided by selected biological tests. J Clin Microbiol 29:2873–2876
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Kwon-Chung K. J. 1976; A new species of Filobasidiella , the sexual state of Cryptococcus neoformans B and C serotypes. Mycologia 68:942–946 [CrossRef]
    [Google Scholar]
  12. Kwon-Chung K. J., Bennett J. E. 1984; Epidemiological differences between the two varieties of Cryptococcus neoformans . Am J Epidemiol 120:123–130
    [Google Scholar]
  13. Kwon-Chung K. J., Bennett J. E., Rhodes J. C. 1982; Taxonomic studies on Filobasidiella species and their anamorphs. Antonie Leeuwenhoek 48:25–38 [CrossRef]
    [Google Scholar]
  14. Kwon-Chung K. J., Sorrell T. C., Dromer F., Fung E., Levitz S. M. 2000; Cryptococcosis: clinical and biological aspects. Med Mycol 38 :Suppl. 1205–213 [CrossRef]
    [Google Scholar]
  15. Lengeler K. B., Cox G. M., Heitman J. 2001; Serotype AD strains of Cryptococcus neoformans are diploid or aneuploid and are heterozygous at the mating-type locus. Infect Immun 69:115–122 [CrossRef]
    [Google Scholar]
  16. Mitchell T. G., Perfect J. R. 1995; Cryptococcosis in the era of AIDS – 100 years after the discovery of Cryptococcus neoformans . Clin Microbiol Rev 8:515–548
    [Google Scholar]
  17. Ponce de Leon G., Sattah M., Graviss E. A., Phelan M., Brandt M. E., Rimland D., Hamill R., Hajjeh R. A. 1999 Cryptococcosis surveillance: population-based trends, Atlanta and Houston, 1993–98. Abstracts of the 37th IDSA meeting, Philadelphia, PA abstract no. 405
    [Google Scholar]
  18. Swofford D. L. 2001 PAUP 4.0: Phylogenetic Analysis Using Parsimony. Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  19. Williamson P. R. 1994; Biochemical and molecular characterization of the diphenol oxidase of Cryptococcus neoformans : identification as a laccase. J Bacteriol 176:656–664
    [Google Scholar]
  20. Xu J., Ali R., Gregory D., Amick D., Lambert S., Yoell H., Vilgalys R., Mitchell T. G. 2000a; Uniparental mitochondrial transmission in sexual crosses in Cryptococcus neoformans . Curr Microbiol 40:269–273 [CrossRef]
    [Google Scholar]
  21. Xu J., Ramos A. R., Vilgalys R. J., Mitchell T. G. 2000b; Clonal and spontaneous origins of fluconazole resistance in Candida albicans . J Clin Microbiol 38:1214–1220
    [Google Scholar]
  22. Xu J., Vilgalys R. J., Mitchell T. G. 2000c; Multiple gene genealogies reveal recent dispersion and hybridization in the human pathogenic fungus Cryptococcus neoformans . Mol Ecol 9:1471–1481 [CrossRef]
    [Google Scholar]
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