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Volume 148, Issue 11

Bovicin HC5, a bacteriocin from HC5 Free

Abstract

Previous work indicated that HC5 had significant antibacterial activity, and even nisin-resistant JB1 cells could be strongly inhibited. HC5 inhibited a variety of Gram-positive bacteria and the spectrum of activity was similar to monensin, a commonly used feed additive. The crude extracts (ammonium sulfate precipitation) were inactivated by Pronase E and trypsin, but the activity was resistant to heat, proteinase K and α-chymotrypsin. Most of the antibacterial activity was cell associated, but it could be liberated by acidic NaCl (100 mM, pH 20) without significant cell lysis. When glycolysing JB1 cells were treated with either crude or acidic NaCl extracts, intracellular potassium declined and this result indicated the antibacterial activity was mediated by a pore-forming peptide. The peptide could be purified by HPLC and matrix-assisted laser desorption ionization time-of-flight analysis indicated that it had a molecular mass of approximately 2440 Da. The terminal amino acid sequence was VGXRYASXPGXSWKYVXF. The unnamed amino acid residues (designated by X) had approximately the same position as dehydroalanines found in some lantibiotics, but samples that were reduced and alkylated prior to Edman degradation did not have cysteine residues. The only other bacteriocin that had significant similarity was the lantibiotic precursor of SF370, but the identity was only 55%. Based on these results, the bacteriocin of HC5 appears to be novel and the authors now designate it as bovicin HC5.

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  • Accepted:
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  • Published Online:
Keyword(s): lactic acid bacteria , MALDI-TOF, matrix-assisted laser desorption ionization time-of-flight , purification, N-terminal amino acids , rumen and TFA, trifluoroacetic acid
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2002-11-01
2024-04-26
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References

  1. Ausubel F, Brent R, Kingston R. E, Moore D. D, Seidman J. G, Smith J. A., Struhl K. 1997 Short Protocols in Molecular Biology, 3rd edn. New York: Wiley;
     [Google Scholar]
  2. Callaway T. R., Russell J. B. 2000; Variations in the ability of ruminal gram-negative Prevotella species to resist monensin. Curr Microbiol 40:185–190
     [Google Scholar]
  3. Callaway T. R, Carneiro De Melo A. M. S., Russell J. B. 1997; The effect of nisin and monensin on ruminal fermentations in vitro. Curr Microbiol 35:90–96
     [Google Scholar]
  4. Carolissen-Mackay V, Arendse G., Hastings J. W. 1997; Purification of bacteriocins of lactic acid bacteria: problems and pointers. Int J Food Microbiol 34:1–16
     [Google Scholar]
  5. Cotta M. A., Russell J. B. 1982; Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture. J Dairy Sci 65:226–234
     [Google Scholar]
  6. De Vos W. M, Kuipers O. P, van der Meer J. R., Siezen R. J. 1995; Maturation pathway of nisin and other lantibiotics: post-translationally modified antimicrobial peptides exported by Gram-positive bacteria. Mol Microbiol 17:427–437
     [Google Scholar]
  7. Ferretti J. J, McShan W. M, Ajdic D. 20 other authors 2001; Complete genome sequence of an M1 strain of Streptococcus pyogenes. Proc Natl Acad Sci USA 98:4658–4663
     [Google Scholar]
  8. Guder A, Wiedemann I., Sahl H.-G. 2000; Post-translationally modified bacteriocins – the lantibiotics. Biopolymers 55:62–73
     [Google Scholar]
  9. Iverson W. G., Millis N. F. 1976; Bacteriocins of Streptococcus bovis . Can J Microbiol 22:1040–1047
     [Google Scholar]
  10. Jack R. W, Tagg J. R., Ray B. 1995; Bacteriocins of Gram-positive bacteria. Microbiol Rev 59:171–200
     [Google Scholar]
  11. Kalmokoff M. L, Bartlett F., Teather R. M. 1996; Are ruminal bacteria armed with bacteriocins?. J Dairy Sci 79:2297–2306
     [Google Scholar]
  12. Mantovani H. C., Russell J. B. 2001; Nisin resistance of Streptococcus bovis . Appl Environ Microbiol 67:808–813
     [Google Scholar]
  13. Mantovani H. C, Kam D. K, Ha J. K., Russell J. B. 2001; The antibacterial activity and sensitivity of Streptococcus bovis strains isolated from the rumen of cattle. FEMS Microbial Ecol 37:223–229
     [Google Scholar]
  14. Nel H. A, Bauer R, Vandamme E. J., Dicks L. M. T. 2001; Growth optimization of Pediococcus damnosus NCFB 1832 and the influence of pH and nutrients on the production of pediocin PD-1. J Appl Microbiol 91:1131–1138
     [Google Scholar]
  15. Parente E., Ricciardi A. 1999; Production, recovery and purification of bacteriocins from lactic acid bacteria. Appl Microbiol Biotechnol 52:628–638
     [Google Scholar]
  16. Pattnaik P, Kaushik J. K, Grover S., Batish V. K. 2001; Purification and characterization of a bacteriocin-like compound (lichenin) produced anaerobically by Bacillus licheniformis isolated from water buffalo. J Appl Microbiol 91:636–645
     [Google Scholar]
  17. Russell J. B., Rychlik J. L. 2001; Factors that alter rumen microbial ecology. Science 292:1119–1122
     [Google Scholar]
  18. Russell J. B., Strobel H. J. 1989; Mini-Review: the effect of ionophores on ruminal fermentation. Appl Environ Microbiol 55:1–6
     [Google Scholar]
  19. Sablon E, Contreras B., Vandamme E. 2000; Antimicrobial peptides of lactic acid bacteria: mode of action, genetics and biosynthesis. Adv Biochem Eng 68:21–60
     [Google Scholar]
  20. Stackebrandt E, Pohla H, Kroppenstedt R, Hippe H., Woese C. R. 1985; 16S rRNA analysis of Sporomusa , Selenomonas , and Megasphaera : on the phylogenetic origin of Gram-positive eubacteria. Arch Microbiol 143:270–276
     [Google Scholar]
  21. Villani F, Pepe O, Mauriello G, Salzano G, Moschetti G., Coppola S. 1995; Antilisterial activity of thermophilin 347, a bacteriocin produced by Streptococcus thermophilus . Int J Food Microbiol 25:179–190
     [Google Scholar]
  22. Whitford M. F, McPherson M. A, Forster R. J., Teather R. M. 2001; Identification of bacteriocin-like inhibitors from rumen Streptococcus spp. and isolation and characterization of bovicin 255. Appl Environ Microbiol 67:569–574
     [Google Scholar]
  23. Yang R, Johnson M., Ray B. 1992; Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Environ Microbiol 58:3355–3359
     [Google Scholar]
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Bovicin HC5, a bacteriocin from Streptococcus bovis HC5
Microbiology 148, 3347 (2002); https://doi.org/10.1099/00221287-148-11-3347
/content/journal/micro/10.1099/00221287-148-11-3347
/content/journal/micro/10.1099/00221287-148-11-3347
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