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Volume 160, Issue 9

Sequence and expression divergence of an ancient duplication of the chaperonin operon in species Free

Abstract

Heat-shock proteins are molecular chaperones essential for protein folding, degradation and trafficking. The human pathogen encodes a copy of the operon in both chromosomes and these genes share <80 % similarity with each other. Comparative genomic analysis was used to determine whether this duplication is prevalent among specifically or in general. Among the complete genome sequences in the database (31 species), seven species contained a copy of in each chromosome, including the human pathogens , and . Phylogenetic analysis of GroEL among the indicated that GroESEL-1 encoded in chromosome I was the ancestral copy and GroESEL-2 in chromosome II arose by an ancient gene duplication event. Interestingly, outside of the within the , chromosomal duplications were rare among the 296 genomes examined; only five additional species contained two or more copies. Examination of the expression pattern of from cells grown under different conditions revealed differential expression between the copies. The data demonstrate that was more highly expressed during growth in exponential phase than and a similar pattern was also found in both and . Overall these data suggest that retention of both copies of in species may confer an evolutionary advantage.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • National Science Foundation (Award DEB-0844409)
  • BOYSCAST
  • University of Delaware
  • University of Delaware
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2014-09-01
2024-04-26
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Sequence and expression divergence of an ancient duplication of the chaperonin groESEL operon in Vibrio species
Microbiology 160, 1953 (2014); https://doi.org/10.1099/mic.0.079194-0
/content/journal/micro/10.1099/mic.0.079194-0
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