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Volume 151, Issue 6

The cell envelope structure and properties of mc155: is there a clue for the unique transformability of the strain? Free

Abstract

is often used as a surrogate host for pathogenic mycobacteria, especially since the isolation of the transformable smooth morphotype strain mc155 from the isogenic rough wild-type strain ATCC 607. Biochemical analysis of the cell envelope components revealed a lack of polar glycolipids, namely the lipooligosaccharides and the polar subfamilies of glycopeptidolipids, in the mc155 strain. In addition, the latter strain differs from its parent by the distribution of various species of glycolipids and phospholipids between the outermost and deeper layers of the cell envelope. The presence of filamentous and rope-like structures at the cell surface of mc155 cells grown in complex media further supported an ultrastructural change in the cell envelope of the mutant. Importantly, a significantly more rapid uptake of the hydrophobic chenodeoxycholate was observed for the mutant compared to wild-type cells. Taken together, these data indicate that the nature of the surface-exposed and envelope constituents is crucial for the surface properties, cell wall permeability and bacterial phenotype, and suggest that the transformable character of the mc155 strain may be in part explained by these profound modifications of its cell envelope.

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Keyword(s): ATCC, American Type Culture Collection , ept, efficient-plasmid-transformation , GPL, glycopeptidolipid , LOS, lipooligosaccharide , MALDI-TOF, matrix-assisted laser-desorption/ionization-time of flight , PE, phosphatidylethanolamine , PG, phosphatidylglycerol , PI, phosphatidylinositol , PIM, phosphatidylinositolmannoside , SXM, surface-exposed material , TAG, triacylglycerol , TDM, trehalose dimycolate and TMM, trehalose monomycolate
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2005-06-01
2024-04-26
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The cell envelope structure and properties of Mycobacterium smegmatis mc2155: is there a clue for the unique transformability of the strain?
Microbiology 151, 2075 (2005); https://doi.org/10.1099/mic.0.27869-0
/content/journal/micro/10.1099/mic.0.27869-0
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