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Volume 151, Issue 12

Identification of genes associated with mutacin I production in using random insertional mutagenesis Free

Abstract

is a major pathogen implicated in dental caries. Its virulence is enhanced by its ability to produce bacteriocins, called mutacins, which inhibit the growth of other Gram-positive bacteria. The goal of this study is to use a random insertional mutagenesis approach to search for genes that are associated with mutacin I production in the virulent strain UA140. A random insertional mutagenesis library consisting of 11 000 clones was constructed and screened for a mutacin-defective phenotype. Mutacin-defective clones were isolated, and their insertion sites were determined by PCR amplification or plasmid rescue followed by sequencing. A total of twenty-five unique genes were identified. These genes can be categorized into the following functional classes: two-component sensory systems, stress responses, energy metabolism and central cellular processes. Several conserved hypothetical proteins with unknown functions were also identified. These results suggest that mutacin I production is stringently controlled by diverse and complex regulatory pathways.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): PTS, phosphoenolpyruvate : sugar phosphotransferase system and TCS, two-component sensory system
SGM
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2005-12-01
2024-04-27
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Identification of genes associated with mutacin I production in Streptococcus mutans using random insertional mutagenesis
Microbiology 151, 3947 (2005); https://doi.org/10.1099/mic.0.28221-0
/content/journal/micro/10.1099/mic.0.28221-0
/content/journal/micro/10.1099/mic.0.28221-0
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